Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Autor(es)

dos Santos, Amanda ; Botelho, Marina Tenorio ; Joviano, Willian Reina ; Gomes, Vicente ; da Silva, Jose Roberto Machado Cunha ; Umbuzeiro, Gisela de Aragao

• Tipo de documento: Artigo Científico
• Fonte: NVERTEBRATE BIOLOG; v. 142, n. 1, p. 11-pg., 2023-03-02.

Resumo

Hemocytes are circulating blood cells that play a crucial function in amphipods and other crustacean immune systems. The hemocytes of the marine tropical amphipod Parhyale hawaiensis have been used for the evaluation of DNA damage and micronuclei, but they have not been characterized in the scientific literature. The aim of this study was to describe the hemolymph cells of P. hawaiensis and study their phagocytotic activity. Basic dyes were used to differentiate the cell types and the presence of lipids. The total hemocyte counts (THCs) and the proportion and sizes of the hemocyte types were determined. Hemolymph was exposed to Escherichia coli for verification of the presence of phagocytosis. Three cell types, all containing lipids, were identified in P. hawaiensis: granulocytes (oval shape, 13.4 x 7.6 mu m), semi-granulocytes (oval shape, 14.1 x 7.2 mu m), and hyalinocytes (round shape, 9.6 x 7.2 mu m). Those three cell types were found in different percentages in males (64.8%, 31.1%, and 4.2%) and females (70.1%, 28.2%, and 1.7%). THCs for males were 9007 +/- 3800 cells per individual and 4695 +/- 1892 cells per individual for females. The cells of E. coli were phagocytized by the hemocytes. Our findings increased the knowledge of hemocytes in P. hawaiensis and is a step forward in using hemocyte-based immune responses as an endpoint in ecotoxicology. (AU)

• Processo FAPESP: 17/16168-9 - Estudo de técnicas para avaliação de efeitos citogenotóxicos e histopatológicos em anfípodes Parhyale hawaiensis alimentados com ração contendo nanopartículas de prata
• Beneficiário: Marina Tenório Botelho
• Modalidade de apoio: Bolsas no Brasil - Doutorado