| Texto completo | |
| Autor(es): Mostrar menos - |
Gaspar, Matheus Z.
;
Yabunaka, Ana C.
;
Silva-Carvalho, Rosimeire
;
Nascimento, Charles U.
;
Brinholi, Rejane B.
;
Silva, Elisangela O.
;
Gerez, Juliana R.
;
Silva Jr, Nelson J.
;
Torres-Bonilla, Kristian A.
;
Hyslop, Stephen
;
Pacagnelli, Francis L.
;
Floriano, Rafael S.
Número total de Autores: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Cardiovascular Toxicology; v. 23, n. 3-4, p. 15-pg., 2023-02-22. |
| Resumo | |
In this work, we examined the action of two South American coralsnake (Micrurus corallinus and Micrurus dumerilii carinicauda) venoms on rat heart function in the absence and presence of treatment with Brazilian coralsnake antivenom (CAV) and varespladib (VPL), a potent phospholipase A(2) inhibitor. Anesthetized male Wistar rats were injected with saline (control) or a single dose of venom (1.5 mg/kg, i.m.) and monitored for alterations in echocardiographic parameters, serum CK-MB levels and cardiac histomorphology, the latter using a combination of fractal dimension and histopathological methods. Neither of the venoms caused cardiac functional alterations 2 h after venom injection; however, M. corallinus venom caused tachycardia 2 h after venom injection, with CAV (given i.p. at an antivenom:venom ratio of 1:1.5, v/w), VPL (0.5 mg/kg, i.p.) and CAV + VPL preventing this increase. Both venoms increased the cardiac lesional score and serum CK-MB levels compared to saline-treated rats, but only the combination of CAV + VPL prevented these alterations, although VPL alone was able to attenuate the increase in CK-MB caused by M. corallinus venom. Micrurus corallinus venom increased the heart fractal dimension measurement, but none of the treatments prevented this alteration. In conclusion, M. corallinus and M. d. carinicauda venoms caused no major cardiac functional alterations at the dose tested, although M. corallinus venom caused transient tachycardia. Both venoms caused some cardiac morphological damage, as indicated by histomorphological analyses and the increase in circulating CK-MB levels. These alterations were consistently attenuated by a combination of CAV and VPL. (AU) | |
| Processo FAPESP: | 16/11344-0 - Remodelação cardíaca de ratos com disfunção cardíaca direita submetidos a treinamento físico preventivo: estudo morfo-funcional e molecular |
| Beneficiário: | Francis Lopes Pacagnelli |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/14191-6 - Potencial ação do inibidor fosfolipase A2 varespladib (LY-315920) sobre os aspectos sistêmicos do envenenamento pela cobra coral Micrurus corallinus em ratos |
| Beneficiário: | Matheus Zanutto Gaspar |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 20/04287-6 - Caracterização estrutural e farmacológica de toxinas isoladas do veneno de Bothrops bilineatus smaragdinus (víbora de floresta) com potencial atividade moduladora da liberação sináptica motora e de funções cardiovasculares |
| Beneficiário: | Rafael Stuani Floriano |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Regular |