| Texto completo | |
| Autor(es): |
de Oliveira, Perla Novais
;
Matias, Fernando
;
Martinez-Andujar, Cristina
;
Martinez-Melgarejo, Purificacion Andrea
;
Prudencio, angela Sanchez
;
Galeano, Esteban
;
Perez-Alfocea, Francisco
;
Carrer, Helaine
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 4, p. 26-pg., 2023-02-01. |
| Resumo | |
Teak (Tectona grandis) is one of the most important wood sources, and it is cultivated in tropical regions with a significant market around the world. Abiotic stresses are an increasingly common and worrying environmental phenomenon because it causes production losses in both agriculture and forestry. Plants adapt to these stress conditions by activation or repression of specific genes, and they synthesize numerous stress proteins to maintain their cellular function. For example, APETALA2/ethylene response factor (AP2/ERF) was found to be involved in stress signal transduction. A search in the teak transcriptome database identified an AP2/ERF gene named TgERF1 with a key AP2/ERF domain. We then verified that the TgERF1 expression is rapidly induced by Polyethylene Glycol (PEG), NaCl, and exogenous phytohormone treatments, suggesting a potential role in drought and salt stress tolerance in teak. The full-length coding sequence of TgERF1 gene was isolated from teak young stems, characterized, cloned, and constitutively overexpressed in tobacco plants. In transgenic tobacco plants, the overexpressed TgERF1 protein was localized exclusively in the cell nucleus, as expected for a transcription factor. Furthermore, functional characterization of TgERF1 provided evidence that TgERF1 is a promising candidate gene to be used as selective marker on plant breeding intending to improve plant stress tolerance. (AU) | |
| Processo FAPESP: | 15/50634-1 - Transformação genética de Tectona grandis visando aumento de produtividade |
| Beneficiário: | Helaine Carrer |
| Modalidade de apoio: | Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE |