Bioglass/collagen scaffolds combined with bone marrow stromal cells on bone healing in an experimental model in cranial defects in rats

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Kido, H. W. ; Gabbai-Armelin, P. R. ; Magri, A. M. P. ; Fernandes, K. R. ; Cruz, M. A. ; Santana, A. F. ; Caliari, H. M. ; Parisi, J. R. ; Avanzi, I. R. ; Daguano, J. K. M. B. ; Granito, R. N. ; Fortulan, C. A. ; Renno, A. C. M.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BIOMATERIALS APPLICATIONS; v. N/A, p. 13-pg., 2023-03-14.

Resumo

This study aimed to develop bone regenerative therapeutic strategies, based on the addition of bone marrow stromal cells (BMSC) on bioglass/collagen (BG/COL) scaffolds. For this purpose, an in vivo study was conducted using tissue response of the BG/COL scaffolds combined with BMSC in a critical-size defects. Wistar rats were submitted to the surgical procedure to perform the cranial critical size bone defects and distributed in four groups (20 animals per group): Control Group (CG) (rats submitted to the cranial bone defect surgery without treatment), Bioglass Group (BG) (rats treated with BG), BG/ COL Group (rats treated with BG/COL) and Bioglass/Collagen and BMSC Group (BG/COL/BMSC) (rats treated with BG/ COL scaffolds enriched with BMSCs). Animals were euthanized 15 and 30 days after surgery. Scanning electron mi-croscopy, histopathological and immunohistochemistry analysis were used. SEM analysis demonstrated that porous scaffolds were obtained, and Col fibers were successfully impregnated to BG matrices. The implantation of the BMSC on BG/COL based scaffolds was effective in stimulating newly bone formation and produced an increased immunoexpression of markers related to the bone repair. These results highlight the potential of BG/COL scaffolds and BMSCs to be used as a therapeutic approach for bone regeneration. (AU)

• Processo FAPESP: 14/23471-1 - Scaffolds de biovidro/colágeno enriquecidos com células-tronco mesenquimais: efeitos sobre o reparo de defeitos críticos em calvária de ratos
• Beneficiário: Hueliton Wilian Kido
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado