Molecular characterization of waterborne protozoa in surface water and sediment in Brazil: a taxonomic survey of ciliated protozoa and their correlation with Giardia duodenalis and Cryptosporidium spp.

Autor(es)

Bonatti, Tais Rondello ; Siqueira-Castro, Isabel Cristina Vidal ; Leal, Diego Averaldo Guiguet ; Durigan, Mauricio ; Dias, Roberto Junio Pedroso ; Franco, Regina Maura Bueno

• Tipo de documento: Artigo Científico
• Fonte: ENVIRONMENTAL MONITORING AND ASSESSMENT; v. 195, n. 4, p. 18-pg., 2023-04-01.

Resumo

The detection of Giardia duodenalis and Cryptosporidium spp. was performed, along with the identification of the ciliated protozoa biodiversity, to evaluate the correlation between these protozoa in freshwater quality monitoring. Water and sediment samples from two sites in the Atibaia River (Campinas, Sao Paulo, Brazil) were collected monthly for 2 years (n = 96). Pathogenic protozoa in water and sediment were detected by using immunomagnetic separation, followed by visualization by immunofluorescence assay (IFA). All positive aliquots in IFA were subjected to DNA extraction and subsequently nested PCR. Qualitative (in vivo observation and silver impregnation) and quantitative (in vivo enumeration) analyses were performed for the ciliated protozoa. Giardia cysts were detected in 62.5% of the surface water samples and Cryptosporidium spp. in 25.0%. In the sediment, cysts were detected in 35.4% samples and oocysts in 16.6%. A total of 57 samples positive for Giardia cysts were subjected to sequencing, 40 of which were harboring G. duodenalis (24 were characterized as sub-assemblage AII). For ciliated protozoa, 73 taxa belonging to 53 genera were identified over the period of the study. These results revealed a high degree of contamination by waterborne protozoa in the main water source which supplies drinking water for more than one million people in Campinas (Sao Paulo), highlighting the need for continuous monitoring of this catchment site. In addition, the present study provides important data regarding the sources of the water body degradation, i.e., fecal contamination of human origin, in addition to the survey of the ciliated protozoa. (AU)

• Processo FAPESP: 11/50244-8 - Biodiversidade e taxonomia da protozofauna presente em amostras de água bruta superficial e sedimento do Rio Atibaia, na cidade de Campinas, São Paulo, Brasil
• Beneficiário: Regina Maura Bueno Franco
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular