Use of Reflectometer Optipen to assess the preventive effect of a sugarcane cystatin on initial dental erosion in vivo

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Pela, Vinicius Taioqui ; Ventura, Talita Mendes Oliveira ; Taira, Even Akemi ; Thomassian, Larissa Tercilia Grizzo ; Brito, Leonardo ; Matuhara, Yan Eiji ; Henrique-Silva, Flavio ; Groisman, Sonia ; Carvalho, Thiago Saads ; Lussi, Adrian ; Buzalaf, Marilia Afonso Rabelo

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS; v. 141, p. 6-pg., 2023-03-17.

Resumo

The sugarcane cystatin 5 (CaneCPI-5) showed protection against erosion and erosive tooth wear (ETW) under several protocols. However, evaluating these conditions in vivo is hard due to the lack of a suitable device. The aim of this study was to use clinically the relative surface reflection intensity (%SRI) by the Reflectometer Optipen to assess the acquired pellicle engineering with CaneCPI-5 rinse for the prevention of initial erosion in vivo. Nine volunteers were distributed in three cross-over phases, according to the rinse used, as follows: 1) Deionized water (negative control); 2) Elmex (R) (800 ppm Sn2+, 500 ppm F-; positive control); 3) 0.1 mg/mL CaneCPI-5. The following experimental steps were performed: Initially, the volunteers received prophylaxis and the initial %SRI was performed. Subsequently, they rinsed with the solutions (10 mL; 1min), followed by the formation of the acquired enamel pellicle (AEP; 120min). After, the erosive challenge with citric acid 1%, pH 2.5 (10 mu L; 10s) was performed (in isolation) on the buccal surface of the maxillary central incisors (right and left). The calcium present in the acid was analyzed by Arsenazo III method. Finally, the final %SRI was performed. Data were analyzed by Kruskal-Wallis/Dunn's tests and Spearman's correlation were used (p < 0.05). For both variables, the negative control led to significantly less protection (lower reflectivity and higher calcium release) in comparison with the other groups. The best protection (higher reflectivity and lower calcium release) was observed for the Elmex (R) and the CaneCPI-5 groups, with no significant differences between them (p < 0.05). There was a significant correlation between both analyzes. The Reflectometer Optipen demonstrated to be a good device to assess clinically. Moreover, CaneCPI-5 rinse proved effective through acquired pellicle engineering against initial erosion in vivo. (AU)

• Processo FAPESP: 17/04857-4 - Engenharia de película adquirida para o controle da erosão dentária: avaliação in situ do potencial protetor de uma nova cistatina derivada da cana-de-açúcar
• Beneficiário: Vinícius Taioqui Pelá
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/26070-1 - Modulação da película adquirida do esmalte e do biofilme para o controle da perda mineral dentária: desvendando mecanismos e possibilitando terapias
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/08437-5 - Engenharia da película adquirida para proteção contra a erosão dentária: uso de um novo refletômetro óptico portátil para avaliar o papel protetor de proteínas/peptídeos
• Beneficiário: Vinícius Taioqui Pelá
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 18/12041-7 - Segurança e eficácia para prevenção da erosão dentária de uma nova cistatina derivada da cana-de-açúcar (CaneCPI-5): estudos in vitro, in situ e in vivo
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Regular