Microbiological profile of root canals indicated for endodontic retreatment due to secondary endodontic infections or for prosthetic reasons

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Godoi-Jr, Ederaldo P. ; Bronzato, Juliana D. ; Francisco, Priscila A. ; Bicego-Pereira, Eloa C. ; Lopes, Erica M. ; Passini, Maicon R. Z. ; de-Jesus-Soares, Adriana ; Almeida, Jose F. A. ; Marciano, Marina A. ; Ferraz, Caio C. R. ; Gomes, Brenda P. F. A.

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 27, n. 5, p. 16-pg., 2023-03-31.

Resumo

ObjectiveThe objective of this study was to evaluate the microbiological profile of root canals indicated for endodontic retreatment due to secondary endodontic infections evidenced by the presence of chronic apical periodontitis (G1) or for prosthetic reasons, without clinical and radiographic signs of endodontic reinfection (G2).MethodsMicrobiological samples were collected from thirty teeth (N=30) out of which 15 were indicated for retreatment due to the presence of chronic apical periodontitis (G1) and 15 were indicated exclusively for prosthetic reasons (G2). Samples were collected from root canals before (S1), after chemomechanical preparation (S2), and after 30 days of intracanal medication composed of calcium hydroxide and 2% chlorhexidine gel (S3). The molecular analysis was performed using Nested-PCR for the detection of 17 bacterial species. The efficacy of each stage of the retreatment in reducing the microbial load was verified by counting colony-forming units (CFU). The statistical analysis considered a significance level of 5%.ResultsThe results showed that bacteria were detected in 100% of the cases in S1, in both groups, by Nested PCR. The most frequently found species in S1 in both groups were Enterococcus faecalis, Fusobacterium nucleatum, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Parvimonas micra. The microbial load of G1 was higher than G2 in the initial samples (S1). Endodontic retreatment was effective in reducing the microbial load in G1 and G2. Statistically significant associations were found between specific bacteria and clinical signs and symptoms.ConclusionIt was concluded that the microbial community present in the root canal of teeth indicated for endodontic retreatment is mixed and heterogeneous. G1 and G2 differ in the number of species per case and microbial load. (AU)

• Processo FAPESP: 19/14448-0 - Microbiomas e aspectos imunobiológicos nas infecções endodônticas
• Beneficiário: Erica Mendes Lopes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/16516-7 - Investigação do conteúdo microbiológico, expressão de metaloproteinases de matriz e suscetibilidade antimicrobiana de bactérias específicas em casos de insucesso endodôntico
• Beneficiário: Priscila Amanda Francisco
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/25090-3 - Análise do conteúdo microbiológico e da família fadA presentes em canais radiculares e lesões periapicais de dentes com infecção secundária e sua relação com os níveis de citocinas pró-inflamatórias e MMPs
• Beneficiário: Juliana Delatorre Bronzato
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 21/13871-6 - Decodificando as correlações da microbiota de dentes indicados ao retratamento endodôntico e a complexa inter-relação entre mobiloma e resistoma em metagenomas de sítios orais
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/14570-0 - Influência do tempo de infecção sobre o perfil microbiológico (bactérias e fungos), citotóxico e inflamatório da comunidade microbiana de infecções endodônticas secundárias/persistentes
• Beneficiário: Ederaldo Pietrafesa de Godoi Junior
• Modalidade de apoio: Bolsas no Brasil - Doutorado