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A restoration and extrapolation iterative method for band-limited fluorescence microscopy image

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Autor(es):
Ponti, Moacir P. ; Mascarenhas, Nelson D. A. ; Suazo, Claudio A. T.
Número total de Autores: 3
Tipo de documento: Artigo Científico
Fonte: 2009 XXII BRAZILIAN SYMPOSIUM ON COMPUTER GRAPHICS AND IMAGE PROCESSING (SIBGRAPI 2009); v. N/A, p. 3-pg., 2007-01-01.
Resumo

Optical microscopic images, especially with a nonconfocal microscope, are fundamentally limited because the optical transfer function (the Fourier transform of the point-spread function) is zero over a region of the spatial-frequency domain. Iterative algorithms were developed for the restoration and extrapolation of diffraction-limited imagery. In this paper we present the effectiveness of an iterative method based on the Richardson-Lucy algorithm for image restoration and a simultaneous modified version of Gerchberg-Papoulis method to extrapolate the spectrum and control the noise amplification. Good convergence stabilization results were achieved and also good numerical results were observed. (AU)

Processo FAPESP: 02/07153-2 - Algoritmos para a reconstrução tomográfica: otimização, restauração, quantificação e aplicação
Beneficiário:Sergio Shiguemi Furuie
Modalidade de apoio: Auxílio à Pesquisa - Temático