ANTIFUNGAL COMPOUND PRODUCED BY THE CASSAVA ENDOPHYTE Bacillus pumilus MAIIIM4A

Autor(es)

Pereira de Melo, Flavia Mandolesi ; Fiore, Marli Fatima ; Beraldo de Moraes, Luiz Alberto ; Silva-Stenico, Maria Estela ; Scramin, Shirlei ; Teixeira, Manoel de Araujo ; de Melo, Itamar Soares

• Tipo de documento: Artigo Científico
• Fonte: CIENTIA AGRICOL; v. 66, n. 5, p. 10-pg., 2009-01-01.

Resumo

In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy. (AU)

• Processo FAPESP: 99/09177-1 - Diversidade de microrganismos endofíticos e seu potencial biotecnológico
• Beneficiário: Itamar Soares de Melo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático