Crystal structure of a soluble decoy receptor IL-22BP bound to interleukin-22

Autor(es)

de Moura, Patricia Ribeiro ; Watanabe, Leandra ; Bleicher, Lucas ; Colau, Didier ; Dumoutier, Laure ; Lemaire, Muriel M. ; Renauld, Jean-Christophe ; Polikarpov, Igor

• Tipo de documento: Artigo Científico
• Fonte: FEBS LETTERS; v. 583, n. 7, p. 6-pg., 2009-04-02.

Resumo

Interleukin-22 (IL-22) plays an important role in the regulation of immune and inflammatory responses in mammals. The IL-22 binding protein (IL-22BP), a soluble receptor that specifically binds IL-22, prevents the IL-22/interleukin-22 receptor 1 (IL-22R1)/interleukin-10 receptor 2 (IL-10R2) complex assembly and blocks IL-22 biological activity. Here we present the crystal structure of the IL-22/IL-22BP complex at 2.75 angstrom resolution. The structure reveals IL-22BP residues critical for IL-22 binding, which were confirmed by site-directed mutagenesis and functional studies. Comparison of IL-22/IL-22BP and IL-22/IL-22R1 crystal structures shows that both receptors display an overlapping IL-22 binding surface, which is consistent with the inhibitory role played by IL-22 binding protein. (AU)

• Processo FAPESP: 06/01534-5 - Estudos das interações dos receptores de hormônios tireoidianos com DNA e as proteínas co-reguladoras utilizando a cristalografia de raios-X
• Beneficiário: Leandra Watanabe
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 06/00182-8 - Biofísica estrutural dos receptores nucleares e proteínas relacionadas
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Temático