| Texto completo | |
| Autor(es): |
de Moura, Patricia Ribeiro
;
Watanabe, Leandra
;
Bleicher, Lucas
;
Colau, Didier
;
Dumoutier, Laure
;
Lemaire, Muriel M.
;
Renauld, Jean-Christophe
;
Polikarpov, Igor
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FEBS LETTERS; v. 583, n. 7, p. 6-pg., 2009-04-02. |
| Resumo | |
Interleukin-22 (IL-22) plays an important role in the regulation of immune and inflammatory responses in mammals. The IL-22 binding protein (IL-22BP), a soluble receptor that specifically binds IL-22, prevents the IL-22/interleukin-22 receptor 1 (IL-22R1)/interleukin-10 receptor 2 (IL-10R2) complex assembly and blocks IL-22 biological activity. Here we present the crystal structure of the IL-22/IL-22BP complex at 2.75 angstrom resolution. The structure reveals IL-22BP residues critical for IL-22 binding, which were confirmed by site-directed mutagenesis and functional studies. Comparison of IL-22/IL-22BP and IL-22/IL-22R1 crystal structures shows that both receptors display an overlapping IL-22 binding surface, which is consistent with the inhibitory role played by IL-22 binding protein. (AU) | |
| Processo FAPESP: | 06/01534-5 - Estudos das interações dos receptores de hormônios tireoidianos com DNA e as proteínas co-reguladoras utilizando a cristalografia de raios-X |
| Beneficiário: | Leandra Watanabe |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 06/00182-8 - Biofísica estrutural dos receptores nucleares e proteínas relacionadas |
| Beneficiário: | Igor Polikarpov |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |