A Validated Reverse Phase HPLC Analytical Method for Quantitation of Glycoalkaloids in Solanum lycocarpum and Its Extracts

Autor(es)

Jorge Tiossi, Renata Fabiane ; Miranda, Mariza Abreu ; Barreto de Sousa, Joao Paulo ; Garcia Praca, Fabiola Silva ; Lopes Badra Bentley, Maria Vitoria ; McChesney, James Dewey ; Bastos, Jairo Kenupp

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY; v. 2012, p. 8-pg., 2012-01-01.

Resumo

Solanum lycocarpum (Solanaceae) is native to the Brazilian Cerrado. Fruits of this species contain the glycoalkaloids solasonine (SN) and solamargine (SM), which display antiparasitic and anticancer properties. A method has been developed for the extraction and HPLC-UV analysis of the SN and SM in different parts of S. lycocarpum, mainly comprising ripe and unripe fruits, leaf, and stem. This analytical method was validated and gave good detection response with linearity over a dynamic range of 0.77-1000.00 mu g mL(-1) and recovery in the range of 80.92-91.71%, allowing a reliable quantitation of the target compounds. Unripe fruits displayed higher concentrations of glycoalkaloids (1.04% +/- 0.01 of SN and 0.69% +/- 0.00 of SM) than the ripe fruits (0.83% +/- 0.02 of SN and 0.60% +/- 0.01 of SM). Quantitation of glycoalkaloids in the alkaloidic extract gave 45.09% +/- 1.14 of SN and 44.37% +/- 0.60 of SM, respectively. (AU)

• Processo FAPESP: 06/59893-0 - Desenvolvimento de metodologia analítica e estudo fitoquímico do extrato hidroalcoólico e da fração volátil das folhas e ramos de Copaifera langsdorffii (Leguminosae: Caesalpinioideae)
• Beneficiário: João Paulo Barreto de Sousa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/57538-1 - Obtencao de extrato padronizado de solanum lycocarpum contendo glicoalcaloides, desenvolvimento de metodologia analitica em clae e de forma farmaceutica de uso topico para avaliacao da atividade antica..
• Beneficiário: Renata Fabiane Jorge Tiossi
• Modalidade de apoio: Bolsas no Brasil - Mestrado