Analysis Delta 9-THC in cosmetics by high performance liquid chromatography with UV-Vis detection

Autor(es)

Teles de Menezes, Matheus Manoel ; de Andrade, Jose Fernando ; de Oliveira, Marcelo Firmino ; Tristao, Heloisa Maria ; Saczk, Adelir Aparecida ; Okumura, Leonardo Luiz

• Tipo de documento: Artigo Científico
• Fonte: BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY; v. 2, n. 8, p. 4-pg., 2012-04-01.

Resumo

A chromatographic methodology for Delta 9-THC analysis in cosmetics is described. The HPLCUV-Vis technique was employed for quantification of Delta 9-THC in the 1.0 to 100 ppm working range using an optimized mobile phase consisting of methanol/water (4:1). In these experimental conditions, Delta 9-THC presented a retention time of 23.7 minutes at the optimized wavelength of 209 nm. The analytical curve presented a linear correlation coefficient of 3.136x10(-6) and the limits of detection and quantification were 0.746 and 2.487 ppm, respectively. This methodology was employed to quantify Delta 9-THC in hemp oil-based cosmetics and it was possible to detect this cannabinol in concentrations ranging from 0.728 to 2.672 ppm. (AU)

• Processo FAPESP: 12/00084-7 - Desenvolvimento de eletrodos quimicamente modificados para a determinação voltamétrica de cocaína, MDMA, LSD e D-9-tetraidrocanabinol em amostras de interesse judicial
• Beneficiário: Marcelo Firmino de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular