Biochemical differentiation of mycelium and yeast forms of Paracoccidioides brasiliensis by Fourier Transform Infrared Spectroscopy

Autor(es)

Castilho, Maiara L. ; Campos, Claudia B. L. ; Matos, Tatiana G. F. ; de Abreu, Geraldo M. A. ; Martin, Airton A. ; Raniero, Leandro ; MahadevanJansen, A ; Petrich, W

• Tipo de documento: Artigo Científico
• Fonte: BIOMEDICAL VIBRATIONAL SPECTROSCOPY V: ADVANCES IN RESEARCH AND INDUSTRY; v. 8219, p. 6-pg., 2012-01-01.

Resumo

Paracoccidioides brasiliensis the etiological agent of paracoccidioidomycosis, is a dimorphic fungus existing as mycelia in the environment (or at 25 degrees C in vitro) and as yeast cells in the human host (or at 37 degrees C in vitro). The most prominent difference between both forms is probably the cell wall polysaccharide, being 1,3-beta-glucan usually found in mycelia and 1,3-alpha-glucan found in yeasts, but a plethora of other differences have already been described. In this work, we performed a Fourier Transform Infrared Spectroscopy analysis to compare the yeast and mycelia forms of P. brasiliensis and found additional biochemical differences. The analysis of the spectra showed that differences were distributed in chemical bonds of proteins, lipids and carbohydrates. (AU)

• Processo FAPESP: 09/09559-5 - Nanossonda de ouro no diagnóstico do Paracoccidioides brasiliensis
• Beneficiário: Leandro José Raniero
• Modalidade de apoio: Auxílio à Pesquisa - Regular