Neospora caninum as causative agent of bovine encephalitis in Brazil

Autor(es)

Albinati Malaguti, Jane Mary ; Cabral, Aline Diniz ; Abdalla, Raisa Pereira ; Salgueiro, Yolanda Oliveira ; Cacciatori Galleti, Nara Thiers ; Okuda, Liria Hiromi ; Sequetin Cunha, Elenice Maria ; Pituco, Edviges Maristela ; Del Fava, Claudia

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA; v. 21, n. 1, p. 7-pg., 2012-01-01.

Resumo

For supporting the Brazilian bovine encephalitis surveillance program this study examined the differential diagnosis of Neospora caninum in central nervous system (CNS) by histological analysis (HE staining), immunohistochemistry (IHC), and nested-PCR using a set of primers from the Nc5 region of the genomic DNA and ITS1 region of the ribosomal DNA. A sample of 302 cattle presenting neurological syndrome and negative for rabies, aged 0 to 18 years, from herds in 10 Brazilian states was evaluated for N caninum from January 2007 to April 2010. All specimens tested negative with IHC and nested-PCR using primers from the ITS I region of ribosomal DNA, while two positive cases (0.66%) were found using primers from the Nc5 region of genomic DNA: a 20 month-old male and a 72 month-old female, both from Sao Paulo State. Only the male presented severe multifocal necrotizing encephalitis associated with mononuclear cell infiltration, a pathognomonic lesion caused by parasites of the family Sarcocystidae, and only this case was associated with N caninum thus representing 0.33% positivity. Future studies should explore the association of IHC and nested-PCR with real-time PCR, a quantitative method that could be standardized for improving the detection of N. caninum in bovine CNS specimens. (AU)

• Processo FAPESP: 09/11366-0 - Gestão sanitária das encefalites em bovinos: Neospora caninum como agente causal
• Beneficiário: Claudia Del Fava
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/05493-7 - Gestão sanitária das encefalites em bovinos: Neospora caninum como agente causal
• Beneficiário: Yolanda Oliveira Salgueiro
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 10/06359-2 - GESTÃO SANITÁRIA DAS ENCEFALITES EM BOVINOS: Neospora caninum COMO AGENTE CAUSAL.
• Beneficiário: Raisa Pereira Abdalla
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico