Immunomodulation of Homeopathic Thymulin 5CH in a BCG-Induced Granuloma Model

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Bonamin, Leoni Villano ; Sato, Cesar ; Neto, Ruggero Zalla ; Morante, Graziela ; Cardoso, Thayna Neves ; de Santana, Fabiana Rodrigues ; Coelho, Cideli de Paula ; Osugui, Lika ; Popi, Ana Flavia ; Perez Hurtado, Elizabeth Cristina ; Mariano, Mario

• Tipo de documento: Artigo Científico
• Fonte: Evidence-based Complementary and Alternative Medicine; v. 2013, p. 15-pg., 2013-01-01.

Resumo

The present study analyzed the immune modulation mechanisms of thymulin 5CH in a granuloma experimental model. Male adult Balb/c mice were inoculated with BCG into the footpad to induce granuloma, which was quantitatively evaluated. The phenotypic characterization of phagocyte, T- and B-lymphocyte populations in the peritoneum, and local lymph node was done by flow cytometry. During all experimental periods, thymulin 5CH and vehicle (control) were given ad libitum to mice, diluted into the drinking water (1.6 x 10(-17) M). After 7 days from inoculation, thymulin-treated mice presented reduction in the number of epithelioid cytokeratine-positive cells (P = 0.0001) in the lesion, in relation to young phagocytes. After 21 days, the differentiation of B1 peritoneal stem cells into phagocytes reached the peak, being higher in thymulin-treated mice (P = 0.0001). Simultaneously, the score of infected phagocytes in the lesion decreased (P = 0.001), and the number of B1-derived phagocytes, CD4+ and CD8+ T lymphocytes in the local lymph node increased in relation to control (P = 0.0001). No difference was seen on the CD25+ Treg cells. The results show that thymulin 5CH treatment is able to improve the granuloma in inflammatory process and the infection remission, by modulating local and systemic phagocyte differentiation. (AU)

• Processo FAPESP: 10/50842-0 - Efeitos da timulina 5cH na lesão granulomatosa induzida por BCG: participação de células B-1
• Beneficiário: Leoni Villano Bonamin
• Modalidade de apoio: Auxílio à Pesquisa - Regular