Deregulation of Annexin-A1 and Galectin-1 Expression in Precancerous Gastric Lesions: Intestinal Metaplasia and Gastric Ulcer

Autor(es)

Teixeira Rossi, Ana Flavia ; Duarte, Marcia Cristina ; Poltronieri, Ayla Blanco ; Valsechi, Marina Curado ; Jorge, Yvana Cristina ; Neto, Dalisio de-Santi ; Rahal, Paula ; Oliani, Sonia Maria ; Silva, Ana Elizabete

• Tipo de documento: Artigo Científico
• Fonte: Mediators of Inflammation; v. 2014, p. 11-pg., 2014-01-01.

Resumo

Objective. Annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) are mediators that play an important role in the inflammatory response and are also associated with carcinogenesis. We investigated mRNA and protein expression in precancerous gastric lesions that participate in the progression cascade to gastric cancer, such as intestinal metaplasia (IM) and gastric ulcer (GU). Methods. Quantitative real-time PCR (qPCR) and immunohistochemical techniques were used to analyze the relative quantification levels (RQ) of ANXA1 and LGALS1 mRNA and protein expression, respectively. Results. Increased relative expression levels of ANXA1 were found in 100% of cases, both in IM (mean RQ = 6.22 +/- 0.06) and in GU (mean RQ = 6.69 +/- 0.10). However, the LGALS1 presented basal expression in both groups (IM: mean RQ = 0.35 +/- 0.07; GU: mean RQ = 0.69 +/- 0.09). Immunohistochemistry revealed significant positive staining for both the AnxA1 and Gal-1 proteins in the epithelial nucleus and cytoplasm as well as in the stroma of the IM and GU groups (P < 0.05) but absence or low immunorectivity in normal mucosa. Conclusion. Our results bring an important contribution by evidencing that both the AnxA1 and Gal-1 anti-inflammatory proteins are deregulated in precancerous gastric lesions, suggesting their involvement in the early stages of gastric carcinogenesis, possibly due to an inflammatory process in the gastric mucosa. (AU)

• Processo FAPESP: 12/15036-8 - Avaliação da expressão gênica e protéica em processos inflamatórios e neoplasias do trato digestório: estômago e colorretal
• Beneficiário: Ana Elizabete Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular