Novel Ruthenium - Gamma-linolenic Acid Complex Inhibits C6 Rat Glioma Cell Proliferation In Vitro and in the Orthotopic C6 Model In Vivo After Osmotic Pump Infusion

Autor(es)

Miyake, Juliano Andreoli ; Benadiba, Marcel ; Ribeiro, Geise ; Silva, Denise De Oliveira ; Colquhoun, Alison

• Tipo de documento: Artigo Científico
• Fonte: ANTICANCER RESEARCH; v. 34, n. 4, p. 11-pg., 2014-04-01.

Resumo

Aim: Gliomas are primary brain tumours. Gamma-linolenic acid (GLA) exerts anti-proliferative effects. Several ruthenium-containing complexes have antiproliferative effects and can be used as adjuvant therapies in cisplatin-resistant cancer. The present study reports on the anti-proliferative properties and effects on tumour morphology of a novel diruthenium-GLA complex (Ru(2)GLA) and its comparison with GLA in the C6 rat glioma model both in vitro and in vivo. Materials and Methods: In vitro and in vivo experiments were performed on C6 glioma rat cells, and in an orthotopic model. Results: Ru(2)GLA (100 mu M) appears to be an inhibitor of C6 rat glioma cell proliferation. The nuclear area of Ru(2)GLA-treated cells was 2.18-times larger than that of control cells, suggesting DNA replication occurred but mitosis was blocked in the G(2)-M phase. Ru(2)GLA (2 mM) inhibited C6 cell proliferation in vivo and the changes in tumor morphology confirm both cellular uptake and collagen fibre-binding in the extracellular matrix. Conclusion: Ru(2)GLA appears to be a low-toxicity drug and a potential candidate for anti-proliferative therapy of glioma. (AU)

• Processo FAPESP: 09/53573-2 - Estudo do efeito biologico e antitumoral de um novo composto dirutenico contendo ibuprofeno in vitro e in vivo
• Beneficiário: Alison Colquhoun
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/60596-8 - Espécies complexas com potencial aplicação em bioinorgânica, catálise, farmacologia e química ambiental: concepção, preparação, caracterização e reatividade
• Beneficiário: Ana Maria da Costa Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/06592-1 - Metalofármacos anti-inflamatórios e antitumorais: reatividade, propriedades e interações com matrizes hospedeiras biocompatíveis
• Beneficiário: Denise de Oliveira Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular