Bovine NR1I3 gene polymorphisms and its association with feed efficiency traits in Nellore cattle

Autor(es)

Alexandre, Pamela A. ; Gomes, Rodrigo C. ; Santana, Miguel H. A. ; Silva, Saulo L. ; Leme, Paulo R. ; Mudadu, Mauricio A. ; Regitano, Luciana C. A. ; Meirelles, Flvio V. ; Ferraz, Jose B. S. ; Fukumasu, Heidge

• Tipo de documento: Artigo Científico
• Fonte: META GENE; v. 2, p. 12-pg., 2014-12-01.

Resumo

The Nuclear receptor 1 family I member 3 (NR1I3), also known as the Constitutive Androstane Receptor (CAR), was initially characterized as a key regulator of xenobiotic metabolism. However, recent biochemical and structural data suggest that NR1I3 is activated in response to metabolic and nutritional stress in a ligand-independent manner. Thus, we prospected the Bovine NR1I3 gene for polymorphisms and studied their association with feed efficiency traits in Nellore cattle. First, 155 purebredNellore bullswere individuallymeasured for Residual Feed Intake (RFI) and the 25 best (High Feed Efficiency group, HFE) and the 25 worst animals (Low Feed Efficiency group, LFE) were selected for DNA extraction. The entire Bovine NR1I3 gene was amplified and polymorphisms were identified by sequencing. Then, one SNP different between HFE and LFE groupswas genotyped in all the 155 animals and in another 288 animals totalizing 443 Nellore bulls genotyped for association of NR1I3 SNPs with feed efficiency traits. We found 24 SNPs in the NR1I3 gene and choose a statistically different SNP between HFE and LFE groups for further analysis. Genotyping of the 155 animals showed a significant associationwithin SNP and RFI (p = 0.04), Residual Intake and BW Gain (p = 0.04) and Dry Matter Intake (p = 0.01). This SNP is located in the 5' flanking promoter region of NR1I3 gene and different alleles alter the binding site for predicted transcriptional factors as HNF4alpha, CREMand c-MYB, leading us to conclude that NR1I3 expression and regulation might be important to feed efficiency. (C) 2014 The Authors. Published by Elsevier B.V. (AU)

• Processo FAPESP: 08/11363-9 - Polimorfismos em genes relacionados à síntese e à degradação protéica intracelular e suas relações com consumo alimentar residual em bovinos da raça Nelore
• Beneficiário: José Bento Sterman Ferraz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/05650-5 - Avaliação nutrigenética do receptor androstano constitutivo (CAR, NR1i3) sobre a eficiência alimentar em bovinos da raça Nelore
• Beneficiário: Heidge Fukumasu
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/04445-6 - Utilização da análise de vídeo imagem, ultra-sonografia em tempo real e infravermelho como ferramentas para determinação da qualidade da carne bovina
• Beneficiário: Saulo da Luz e Silva
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores