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The Triterpenoid Betulin Protects against the Neuromuscular Effects of Bothrops jararacussu Snake Venom In Vivo

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Ferraz, Miriele Cristina ; de Oliveira, Jhones Luiz ; de Oliveira Junior, Joel Reis ; Cogo, Jose Carlos ; dos Santos, Marcio Galdino ; Franco, Luiz Madaleno ; Puebla, Pilar ; Ferraz, Helena Onishi ; Ferraz, Humberto Gomes ; Teixeira da Rocha, Marisa Maria ; Hyslop, Stephen ; San Feliciano, Arturo ; Oshima-Franco, Yoko
Número total de Autores: 13
Tipo de documento: Artigo Científico
Fonte: Evidence-based Complementary and Alternative Medicine; v. 2015, p. 10-pg., 2015-01-01.
Resumo

We confirmed the ability of the triterpenoid betulin to protect against neurotoxicity caused by Bothrops jararacussu snake venom in vitro in mouse isolated phrenic nerve-diaphragm (PND) preparations and examined its capability of in vivo protection using the rat external popliteal/sciatic nerve-tibialis anterior (EPSTA) preparation. Venom caused complete, irreversible blockade in PND (40 mu g/mL), but only partial blockade (similar to 30%) in EPSTA (3.6mg/kg, i.m.) after 120 min. In PND, preincubation of venom with commercial bothropic antivenom (CBA) attenuated the venom-induced blockade, and, in EPSTA, CBA given i.v. 15 min after venom also attenuated the blockade (by similar to 70% in both preparations). Preincubation of venom with betulin (200 mu g/mL) markedly attenuated the venom-induced blockade in PND; similarly, a single dose of betulin (20mg, i.p., 15 min after venom) virtually abolished the venom-induced decrease in contractility. Plasma creatine kinase activity was significantly elevated 120 min after venom injection in the EPSTA but was attenuated by CBA and betulin. These results indicate that betulin given i.p. has a similar efficacy as CBA given i.v. in attenuating the neuromuscular effects of B. jararacussu venom in vivo and could be a useful complementary measure to antivenom therapy for treating snakebite. (AU)

Processo FAPESP: 08/11005-5 - Dipteryx alata Vogel: estudos da ação antiofídica e de toxicidade
Beneficiário:Yoko Oshima Franco
Modalidade de apoio: Bolsas no Exterior - Pesquisa
Processo FAPESP: 07/53883-6 - Dipteryx alata vogel: estudo da acao antiofidica e da toxicidade.
Beneficiário:Yoko Oshima Franco
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/52643-4 - Estudo da capacidade neutralizante do extrato hidroalcoolico de camellia sinensis (cha verde) contra os efeitos neurotoxico e miotoxico da bothropstoxina-i (bothrops jararacussu).
Beneficiário:Yoko Oshima Franco
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/50669-6 - RTI - 2008 - Plano de aplicação de recursos - reserva técnica para custos de infra-estrutura institucional para pesquisa
Beneficiário:Yoko Oshima Franco
Modalidade de apoio: Auxílio à Pesquisa - Reserva Técnica para Infraestrutura Institucional de Pesquisa
Processo FAPESP: 04/09705-8 - Investigacao farmacologica da atividade antipeconhenta de fracoes obtidas de extratos de casearia sylvestris sw.
Beneficiário:Yoko Oshima Franco
Modalidade de apoio: Auxílio à Pesquisa - Regular