Interleukin-18 Increases TLR4 and Mannose Receptor Expression and Modulates Cytokine Production in Human Monocytes

Autor(es)

Dias-Melicio, Luciane Alarcao ; Fernandes, Reginaldo Keller ; Rodrigues, Daniela Ramos ; Golim, Marjorie Assis ; Victoriano Campos Soares, Angela Maria

• Tipo de documento: Artigo Científico
• Fonte: Mediators of Inflammation; v. 2015, p. 9-pg., 2015-01-01.

Resumo

Interleukin-18 is a proinflammatory cytokine belonging to the interleukin-1 family of cytokines. This cytokine exerts many unique biological and immunological effects. To explore the role of IL-18 in inflammatory innate immune responses, we investigated its impact on expression of two toll-like receptors (TLR2 and TLR4) and mannose receptor (MR) by human peripheral blood monocytes and its effect on TNF-alpha, IL-12, IL-15, and IL-10 production. Monocytes from healthy donors were stimulated or not with IL-18 for 18 h, and then the TLR2, TLR4, and MR expression and intracellular TNF-alpha, IL-12, and IL-10 production were assessed by flow cytometry and the levels of TNF-alpha, IL-12, IL-15, and IL-10 in culture supernatants were measured by ELISA. IL-18 treatment was able to increase TLR4 and MR expression by monocytes. The production of TNF-alpha and IL-10 was also increased by cytokine treatment. However, IL-18 was unable to induce neither IL-12 nor IL-15 production by these cells. Taken together, these results show an important role of IL-18 on the early phase of inflammatory response by promoting the expression of some pattern recognition receptors (PRRs) that are important during the microbe recognition phase and by inducing some important cytokines such as TNF-alpha and IL-10. (AU)

• Processo FAPESP: 07/00755-0 - Papel da il-18 na interacao de monocitos humanos com o paracoccidioides brasiliensis: modulacao de receptores tlr2 e tlr4, inducao de atividade fungicida e modulacao do metabolismo oxidativo.
• Beneficiário: Luciane Alarcão Dias-Melicio
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/04042-9 - Papel da IL-18 na interação de monócitos humanos com o Paracoccidioides brasiliensis: modulação de receptores TLR2 e TLR4, indução de atividade fungicida e modulação do metabolismo oxidativo
• Beneficiário: Angela Maria Victoriano de Campos Soares
• Modalidade de apoio: Auxílio à Pesquisa - Regular