The Effect of Essential Oils and Bioactive Fractions on Streptococcus mutans and Candida albicans Biofilms: A Confocal Analysis

Autor(es)

Freires, Irlan Almeida ; Bueno-Silva, Bruno ; de Carvalho Galvao, Livia Camara ; Teixeira Duarte, Marta Cristina ; Sartoratto, Adilson ; Figueira, Glyn Mara ; de Alencar, Severino Matias ; Rosalen, Pedro Luiz

• Tipo de documento: Artigo Científico
• Fonte: Evidence-based Complementary and Alternative Medicine; v. 2015, p. 9-pg., 2015-01-01.

Resumo

The essential oils (EO) and bioactive fractions (BF) from Aloysia gratissima, Baccharis dracunculifolia, Coriandrum sativum, Cyperus articulatus, and Lippia sidoides were proven to have strong antimicrobial activity on planktonic microorganisms; however, little is known about their effects on the morphology or viability of oral biofilms. Previously, we determined the EO/fractions with the best antimicrobial activity against Streptococcus mutans and Candida spp. In this report, we used a confocal analysis to investigate the effect of these EO and BF on the morphology of S. mutans biofilms (thickness, biovolume, and architecture) and on the metabolic viability of C. albicans biofilms. The analysis of intact treated S. mutans biofilms showed no statistical difference for thickness in all groups compared to the control. However, a significant reduction in the biovolume of extracellular polysaccharides and bacteria was observed for A. gratissima and L. sidoides groups, indicating that these BF disrupt biofilm integrity and may have created porosity in the biofilm. This phenomenon could potentially result in a weakened structure and affect biofilm dynamics. Finally, C. sativum EO drastically affected C. albicans viability when compared to the control. These results highlight the promising antimicrobial activity of these plant species and support future translational research on the treatment of dental caries and oral candidiasis. (AU)

• Processo FAPESP: 11/15984-0 - Avaliação da atividade anti-Candida, antiproliferativa e citotóxica do óleo essencial e frações de Coriandrum sativum L.
• Beneficiário: Irlan de Almeida Freires
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/12353-0 - Avaliação da atividade antimicrobiana de óleos essenciais de plantas contra microrganismos do grupo mutans
• Beneficiário: Lívia Câmara de Carvalho Galvão
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/14757-0 - Avaliação da atividade antimicrobiana de óleos essenciais sobre patógenos bucais
• Beneficiário: Pedro Luiz Rosalen
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/01500-4 - Avaliação dos efeitos da própolis vermelha e seus compostos bioativos na modulação da resposta de macrófagos
• Beneficiário: Bruno Bueno Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado