| Texto completo | |
| Autor(es): |
Jimenez, Diego Javier
;
Dini-Andreote, Francisco
;
Ottoni, Julia Ronzella
;
de Oliveira, Valeria Maia
;
van Elsas, Jan Dirk
;
Andreote, Fernando Dini
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MICROBIAL BIOTECHNOLOGY; v. 8, n. 3, p. 10-pg., 2015-05-01. |
| Resumo | |
The occurrence of genes encoding biotechnologically relevant /-hydrolases in mangrove soil microbial communities was assessed using data obtained by whole-metagenome sequencing of four mangroves areas, denoted BrMgv01 to BrMgv04, in SAo Paulo, Brazil. The sequences (215Mb in total) were filtered based on local amino acid alignments against the Lipase Engineering Database. In total, 5923 unassembled sequences were affiliated with 30 different /-hydrolase fold superfamilies. The most abundant predicted proteins encompassed cytosolic hydrolases (abH08;approximate to 23%), microsomal hydrolases (abH09;approximate to 12%) and Moraxella lipase-like proteins (abH04 and abH01; <5%). Detailed analysis of the genes predicted to encode proteins of the abH08 superfamily revealed a high proportion related to epoxide hydrolases and haloalkane dehalogenases in polluted mangroves BrMgv01-02-03. This suggested selection and putative involvement in local degradation/detoxification of the pollutants. Seven sequences that were annotated as genes for putative epoxide hydrolases and five for putative haloalkane dehalogenases were found in a fosmid library generated from BrMgv02 DNA. The latter enzymes were predicted to belong to Actinobacteria, Deinococcus-Thermus, Planctomycetes and Proteobacteria. Our integrated approach thus identified 12 genes (complete and/or partial) that may encode hitherto undescribed enzymes. The low amino acid identity (<60%) with already-described genes opens perspectives for both production in an expression host and genetic screening of metagenomes. (AU) | |
| Processo FAPESP: | 04/13910-6 - Biodiversidade e atividades funcionais de microrganismos de manguezais do estado de São Paulo |
| Beneficiário: | Itamar Soares de Melo |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Temático |
| Processo FAPESP: | 12/06245-2 - Metatranscriptômica e contexto genômico de genes microbianos relacionados aos ciclos biogeoquímicos em manguezais |
| Beneficiário: | Fernando Dini Andreote |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/50809-5 - Prospecção de metagenoma microbiano de sedimentos de manguezais na busca por novos compostos bioativos |
| Beneficiário: | Valeria Maia Merzel |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Regular |
| Processo FAPESP: | 10/51981-3 - Prospeccao e analise funcional de enzimas provenientes de microbiota de manguezais do estado de sao paulo. |
| Beneficiário: | Júlia Ronzella Ottoni |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |