Modulation of Th1/Th2 Immune Responses by Killed Propionibacterium acnes and Its Soluble Polysaccharide Fraction in a Type I Hypersensitivity Murine Model: Induction of Different Activation Status of Antigen-Presenting Cells

Autor(es)

Squaiella-Baptistao, Carla Cristina ; Teixeira, Daniela ; Mussalem, Juliana Sekeres ; Ishimura, Mayari Eika ; Longo-Maugeri, Ieda Maria

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF IMMUNOLOGY RESEARCH; v. 2015, p. 14-pg., 2015-01-01.

Resumo

Propionibacterium acnes (P. acnes) is a gram-positive anaerobic bacillus present in normal human skin microbiota, which exerts important immunomodulatory effects, when used as heat-or phenol-killed suspensions. We previously demonstrated that heat-killed P. acnes or its soluble polysaccharide (PS), extracted from the bacterium cell wall, suppressed or potentiated the Th2 response to ovalbumin (OVA) in an immediate hypersensitivity model, depending on the treatment protocol. Herein, we investigated the mechanisms responsible for these effects, using the same model and focusing on the activation status of antigen-presenting cells (APCs). We verified that higher numbers of APCs expressing costimulatory molecules and higher expression levels of these molecules are probably related to potentiation of the Th2 response to OVA induced by P. acnes or PS, while higher expression of toll-like receptors (TLRs) seems to be related to Th2 suppression. In vitro cytokines production in cocultures of dendritic cells and T lymphocytes indicated that P. acnes and PS seem to perform their effects by acting directly on APCs. Our data suggest that P. acnes and PS directly act on APCs, modulating the expression of costimulatory molecules and TLRs, and these differently activated APCs drive distinct T helper patterns to OVA in our model. (AU)

• Processo FAPESP: 04/10354-5 - Modulacao da propionibacterium acnes e sua fracao polissacaridica soluvel sobre celulas dendriticas e linfocitos b1 em um modelo murino de hipersensibilidade tipo 1.
• Beneficiário: Carla Cristina Squaiella
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/23593-0 - Modulação das respostas imunes Th1/Th2 pela Propionibacterium acnes morta e sua fração polissacarídica solúvel em um modelo murino de hipersensibilidade tipo I: indução de diferentes estados de ativação de células apresentadoras de antígenos
• Beneficiário: Carla Cristina Squaiella
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo