| Texto completo | |
| Autor(es): |
Soares Melo, Stephano Freitas
;
Barauna, Valerio Garrone
;
Carneiro Junior, Miguel Araujo
;
Marchesi Bozi, Luiz Henrique
;
Drummond, Lucas Rios
;
Natali, Antonio Jose
;
de Oliveira, Edilamar Menezes
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 16, n. 4, p. 13-pg., 2015-04-01. |
| Resumo | |
Aims: To determine the effects of resistance training (RT) on the expression of microRNA (miRNA)-214 and its target in sarcoplasmic reticulum Ca2+-ATPase (SERCA2a), and on the morphological and mechanical properties of isolated left ventricular myocytes. Main methods: Male Wistar rats were divided into two groups (n = 7/group): Control (CO) or trained (TR). The exercise-training protocol consisted of: 4 x 12 bouts, 5x/week during 8 weeks, with 80% of one repetition maximum. Key findings: RT increased the left ventricular myocyte width by 15% and volume by 12%, compared with control animals (p < 0.05). The time to half relaxation and time to peak were 8.4% and 4.4% lower, respectively, in cells from TR group as compared to CO group (p < 0.05). RT decreased miRNA-214 level by 18.5% while its target SERCA2a expression were 18.5% higher (p < 0.05). Significance: Our findings showed that RT increases single left ventricular myocyte dimensions and also leads to faster cell contraction and relaxation. These mechanical adaptations may be related to the augmented expression of SERCA2a which, in turn, may be associated with the epigenetic modification of decreased miRNA-214 expression. (AU) | |
| Processo FAPESP: | 10/09438-0 - UTILIZAÇÃO TERAPÊUTICA DE microRNAS RESPONSÁVEIS PELA REGULAÇÃO DA CONTRATILIDADE CARDÍACA ASSOCIADO AO TREINAMENTO FÍSICO AERÓBICO EM RATOS COM INSUFICIÊNCIA CARDÍACA |
| Beneficiário: | Stéphano Freitas Soares Melo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 09/18370-3 - EXPRESSÃO DE microRNAS NO CORAÇÃO DE RATOS NORMOTENSOS SUBMETIDOS A DIFERENTES VOLUMES DE TREINAMENTO FÍSICO AERÓBICO E SEU POTENCIAL TERAPÊUTICO NA HIPERTENSÃO |
| Beneficiário: | Edilamar Menezes de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 10/50048-1 - Bases celulares e funcionais do exercício físico na doença cardiovascular |
| Beneficiário: | Carlos Eduardo Negrão |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |