Busca avançada
Ano de início
Entree


Molecular phylogeny of Toxoplasmatinae: comparison between inferences based on mitochondrial and apicoplast genetic sequences

Texto completo
Autor(es):
Sercundes, Michelle Klein ; Oshiro Branco Valadas, Samantha Yuri ; Keid, Lara Borges ; Ferreira Souza Oliveira, Tricia Maria ; Ferreira, Helena Lage ; de Almeida Vitor, Ricardo Wagner ; Gregori, Fabio ; Soares, Rodrigo Martins
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA; v. 25, n. 1, p. 8-pg., 2016-01-01.
Resumo

Phylogenies within Toxoplasmatinae have been widely investigated with different molecular markers. Here, we studied molecular phylogenies of the Toxoplasmatinae subfamily based on apicoplast and mitochondrial genes. Partial sequences of apicoplast genes coding for caseinolytic protease (clpC) and beta subunit of RNA polymerase (rpoB), and mitochondrial gene coding for cytochrome B (cytB) were analyzed. Laboratory-adapted strains of the closely related parasites Sarcocystis falcatula and Sarcocystis neurona were investigated, along with Neospora caninum, Neospora hughesi, Toxoplasma gondii (strains RH, CTG and PTG), Besnoitia akodoni, Hammondia hammondi and two genetically divergent lineages of Hammondia heydorni. The molecular analysis based on organellar genes did not clearly differentiate between N. caninum and N. hughesi, but the two lineages of H. heydorni were confirmed. Slight differences between the strains of S. falcatula and S. neurona were encountered in all markers. In conclusion, congruent phylogenies were inferred from the three different genes and they might be used for screening undescribed sarcocystid parasites in order to ascertain their phylogenetic relationships with organisms of the family Sarcocystidae. The evolutionary studies based on organelar genes confirm that the genus Hammondia is paraphyletic. The primers used for amplification of clpC and rpoB were able to amplify genetic sequences of organisms of the genus Sarcocystis and organisms of the subfamily Toxoplasmatinae as well. (AU)

Processo FAPESP: 07/56836-9 - Filogenia molecular de protozoários pertencentes à sub-familia Toxoplasmatinae pela análise de genes mitocondriais e de apicoplasto
Beneficiário:Michelle Klein Sercundes
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 11/10550-2 - Caracterização molecular de Sarcocystis spp. obtidos de fezes de marsupiais do gênero Didelphis spp. pela análise de genes mitocondriais, genes de apicoplasto, espaçadores internos transcritos (ITS-1) e genes codificadores de antígenos de superfície
Beneficiário:Samantha Yuri Oshiro Branco Valadas
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto