| Texto completo | |
| Autor(es): |
Carbayo, Fernando
;
Lenihan, Jennifer Winifred
Número total de Autores: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | GIGASCIENCE; v. 5, p. 6-pg., 2016-03-16. |
| Resumo | |
Background: We investigated whether images obtained through X-ray micro-computed tomography (mu CT) can be used in conjunction with traditional methods for morphological studies of soft-bodied land planarians. mu CT is non-invasive and provides true-to-scale three-dimensional imagery at high resolution. We compared mu CT-based images of a recently described land planarian species of Obama otavioi (Platyhelminthes) with those obtained from light microphotography of histological sections, most of which were also digitized at high magnification. Findings: The specimens studied were collected in 2012. Subsequent mu CT-based images of the stained body of a paratype show nearly all morphological features provided by traditional histology, with the exception of particularly minute structures, smaller than 5 mu m, such as the sensory pits and single muscle fibers, which are best visible on traditional histological sections. Because the technique is non-destructive, the scanned specimen is preserved without damage. The raw and derivative mu CT data and virtual histological sections are freely available in GigaDB. Conclusions: The mu CT datasets of these stained soft-bodied organisms reveal images of external and internal structures that support previous taxonomic studies. This technique can be particularly important for non-destructively revealing internal details of whole museum specimens at a faster rate than histology alone. High-resolution virtual histological slides also allow further searches for new, previously unstudied morphological features. The use of X-ray equipment with higher resolution can enable smaller sensory organ and muscle fiber details to be seen. The image sets, mu CT-based images and digitized histological slides can be disseminated without the constraints of specimen loans. (AU) | |
| Processo FAPESP: | 14/13661-8 - Revisão taxonômica das planárias terrestres de Geoplana (Platyhelminthes, Tricladida) |
| Beneficiário: | Fernando Jesús Carbayo Baz |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |