Autor(es)

Freitas Filho, Edismauro Garcia ; Marcelino da Silva, Elaine Zayas ; Zanotto, Camila Ziliotto ; Oliver, Constance ; Jamur, Andmaria Celia

• Tipo de documento: Artigo Científico
• Fonte: Mediators of Inflammation; v. 2016, p. 10-pg., 2016-01-01.

Resumo

Mast cells are immunoregulatory cells that participate in inflammatory processes. Cross-linking mast cell specific GD1b derived gangliosides by mAbAA4 results in partial activation of mast cells without the release of preformed mediators. The present study examines the release of newly formed and newly synthesized mediators following ganglioside cross-linking. Cross-linking the gangliosides with mAbAA4 released the newly formed lipid mediators, prostaglandins D-2 and E-2, without release of leukotrienes B-4 and C 4. The effect of cross-linking these gangliosides on the activation of enzymes in the arachidonate cascade was then investigated. Ganglioside cross-linking resulted in phosphorylation of cytosolic phospholipase A(2) and increased expression of cyclooxygenase-2. Translocation of 5-lipoxygenase from the cytosol to the nucleus was not induced by ganglioside cross-linking. Cross-linking of GD1b derived gangliosides also resulted in the release of the newly synthesized mediators, interleukin-4, interleukin-6, and TNF-alpha. The effect of cross-linking the gangliosides on the MAP kinase pathway was then investigated. Cross-linking the gangliosides induced the phosphorylation of ERK1/2, JNK1/2, and p38 as well as activating both NF kappa B and NFAT in a Syk-dependent manner. Therefore, cross-linking the mast cell specific GD1b derived gangliosides results in the activation of signaling pathways that culminate with the release of newly formed and newly synthesized mediators. (AU)

• Processo FAPESP: 14/17671-8 - Gangliosídios específicos de mastócitos como moduladores do processo de liberação de mediadores
• Beneficiário: Maria Célia Jamur
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/12861-0 - O papel de gangliosídeos específicos como moduladores da liberação de mediadores de mastócitos
• Beneficiário: Edismauro Garcia Freitas Filho
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/06373-0 - O envolvimento do complexo adaptador AP-3 na secreção regulada em mastócitos
• Beneficiário: Elaine Zayas Marcelino da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado