Osteogenic potential of periodontal ligament stem cells are unaffected after exposure to lipopolysaccharides

Autor(es)

Albiero, Mayra Laino ; Amorim, Bruna Rabelo ; Casati, Myrcio Zaffalon ; Sallum, Enilson Antonio ; Nociti Junior, Francisco Humberto ; Silverio, Karina Gonzales

• Tipo de documento: Artigo Científico
• Fonte: BRAZILIAN ORAL RESEARCH; v. 31, p. 10-pg., 2017-01-01.

Resumo

Periodontitis develops as a result of a continuous interaction between host cells and subgingival pathogenic bacteria. The periodontium has a limited capacity for regeneration, probably due to changes in periodontal ligament stem cells (PDLSCs) phenotype. The aim of this study was to evaluate the effects of lipopolysaccharides from Porphyromonas gingivalis (PgLPS) on mesenchymal phenotype and osteoblast/cementoblast (O/C) potential of PDLSCs. PDLSCs were assessed for Toll-like receptor 2 (TLR2) expression by immunostaining technique. After, cells were exposed to PgLPS, and the following assays were carried out: (i) cell metabolic activity using MTS; (ii) gene expression for IL-1 beta, TNF-alpha and OCT-4 by real-time polymerase chain reaction (RT-qPCR); (iii) flow cytometry for STRO-1 and CD105, and (iv) osteogenic differentiation. PDLSCs were positive for TLR2. PgLPS promoted cell proliferation, produced IL-1 beta and TNF-alpha, and did not affect the expression of stem cell markers, STRO-1, CD105 and OCT-4. Under osteogenic condition, PDLSCs exposed to PgLPS showed a similar potential to differentiate toward osteoblast/cementoblast phenotype compared to control group as revealed by mineralized matrix deposition and levels of transcripts for RUNX2, ALP and OCN. These results provide evidence that PgLPS induces pro-inflammatory cytokines, but does not change the mesenchymal phenotype and osteoblast/cementoblast differentiation potential of PDLSCs. (AU)

• Processo FAPESP: 11/04757-3 - Efeito do LPS da porphyromonas gingivalis nas propriedades biológicas das células mesenquimais indiferenciadas do ligamento periodontal de humanos
• Beneficiário: Mayra Laino Albiero
• Modalidade de apoio: Bolsas no Brasil - Mestrado