| Texto completo | |
| Autor(es): |
Pereira Figueiredo, Mayra Araguaia
;
Di Santi, Silvia Maria
;
Manrique, Wilson Gomez
;
Andre, Marcos Rogerio
;
Machado, Rosangela Zacarias
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA; v. 27, n. 3, p. 14-pg., 2018-07-01. |
| Resumo | |
The aim of this study was to identify Plasmodium spp. in blood samples from nonhuman primates (NHPs) in the state of Maranhao, using classical and alternative techniques for examination of human malaria. A total of 161 blood samples from NHPs were analyzed: 141 from captive animals at a Wildlife Screening Center (CETAS) and 20 from free-living animals in a private reserve. The techniques used were microscopy, rapid diagnostic test (RDT), Indirect fluorescent antibody test (IFAT) and molecular techniques (semi-nested PCR, quantitative real-time PCR and LAMP). Two serological methods (dot-ELISA and indirect ELISA) were also standardized with rhoptry protein-soluble antigen of P. falciparum and P. berghei. Trophozoite forms of Plasmodium sp. were identified on slides from five different animals. No samples were positive through RDT and LAMP. Four samples were seropositive for P. malariae through IFAT. The samples showed low reactivity to ELISA. Plasmodium sp. was detected in 34.16% (55/161) of the samples using qPCR based on the 18S rRNA gene. After sequencing, two samples showed 100% identityl to P. malariae, one showed 97% identity to Plasmodium sp. ZOOBH and one showed 99% identity to P. falciparum. PCR was shown to be the most sensitive technique for diagnosing Plasmodium in NHP samples. (AU) | |
| Processo FAPESP: | 12/03961-9 - IDENTIFICAÇÃO DE Plasmodium spp. EM PRIMATAS NEOTROPICAIS E EM ANOFELINOS EM MUNICIPIOS DA ILHA DE SÃO LUIS E NO MUNICÍPIO DE IMPERATRIZ, ESTADO DO MARANHÃO. |
| Beneficiário: | Mayra Araguaia Pereira Figueiredo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 10/12820-4 - Caracterização soroepidemiológica e molecular da infecção malárica em primatas neotropicais no estado do Maranhão |
| Beneficiário: | Rosangela Zacarias Machado |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |