Effects of Diacetyl Flavoring Exposure in Mice Metabolism

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Lima Jedlicka, Leticia Dias ; Silva, Juciara da Costa ; Balbino, Aleksandro Martins ; Neto, Giuseppe Bruno ; Sousa Furtado, Danielle Zildeana ; Torres da Silva, Heron Dominguez ; Correia Cavalcanti, Fernandade Barros ; van der Heijden, Karin Marie ; Avellaneda Penatti, Carlos Alberto ; Henriques Bechara, Etelvino Jose ; Assuncao, Nilson Antonio

• Tipo de documento: Artigo Científico
• Fonte: BIOMED RESEARCH INTERNATIONAL; v. 2018, p. 11-pg., 2018-01-01.

Resumo

Diacetyl is a flavoring that imparts a buttery flavor to foods, but the use or exposure to diacetyl has been related to some diseases. We investigated the effect of oral intake of diacetyl in male and female C57/Bl mice. We performed a target metabolomics assay using ultraperformance liquid chromatography paired with triple quadrupole mass spectrometry (UPLC-MS/MS) for the determination and quantification of plasmatic metabolites. We observed alterations in metabolites present in the urea and tricarboxylic acid (TCA) cycles. Peroxynitrite plasmatic levels were evaluated by a colorimetric method, final activity of superoxide dismutase (SOD) was evaluated by an enzymatic method, and mouse behavior was evaluated. Majority of the assay showed differences between control and treatment groups, as well as between genders. This may indicate the involvement of sex hormones in the regulation of a normal metabolic profile, and the implication of sex differences in metabolite disease response. (AU)

• Processo FAPESP: 13/07763-0 - Estudos da Acetilação de Proteínas Via Radicalar Empregando Ferramentas Proteomicas
• Beneficiário: Leticia Dias Lima Jedlicka
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/02385-0 - Correlação entre os Perfis Metabolômico, Lipidômico e Proteômico e o Tamanho da Deleção 5p de Portadores da Síndrome de Cri-Du-Chat
• Beneficiário: Nilson Antonio de Assunção
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/02514-9 - Uso de técnicas proteômicas para o estudo da acetilação de proteínas via radicalar desencadeada pelos produtos da reação diacetil/peroxinitrito
• Beneficiário: Nilson Antonio de Assunção
• Modalidade de apoio: Auxílio à Pesquisa - Regular