Metarhizium robertsii illuminated during mycelial growth produces conidia with increased germination speed and virulence

Autor(es)

Oliveira, Ariel S. ; Braga, Gilberto U. L. ; Rangel, Drauzio E. N.

• Tipo de documento: Artigo Científico
• Fonte: FUNGAL BIOLOGY; v. 122, n. 6, p. 8-pg., 2018-06-01.

Resumo

Light conditions during fungal growth are well known to cause several physiological adaptations in the conidia produced. In this study, conidia of the entomopathogenic fungi Metarhizium robertsii were produced on: 1) potato dextrose agar (PDA) medium in the dark; 2) PDA medium under white light (4.98 W m(-2)); 3) PDA medium under blue light (4.8 W m(-2)); 4) PDA medium under red light (2.8 W m(-2)); and 5) minimum medium (Czapek medium without sucrose) supplemented with 3 % lactose (MML) in the dark. The conidial production, the speed of conidial germination, and the virulence to the insect Tenebrio molitor (Coleoptera: Tenebrionidae) were evaluated. Conidia produced on MML or PDA medium under white or blue light germinated faster than conidia produced on PDA medium in the dark. Conidia produced under red light germinated slower than conidia produced on PDA medium in the dark. Conidia produced on MML were the most virulent, followed by conidia produced on PDA medium under white light. The fungus grown under blue light produced more conidia than the fungus grown in the dark. The quantity of conidia produced for the fungus grown in the dark, under white, and red light was similar. The MML afforded the least conidial production. In conclusion, white light produced conidia that germinated faster and killed the insects faster; in addition, blue light afforded the highest conidial production. (C) 2017 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 16/11386-5 - Estudo mecanístico da percepção e da resposta a luz visível no fungo entomopatogênico Metarhizium acridum
• Beneficiário: Gilberto Úbida Leite Braga
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/15204-8 - Estudo mecanístico da inativação fotodinâmica de fungos patógenos de humanos e de fungos fitopatógenos
• Beneficiário: Gilberto Úbida Leite Braga
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/50518-6 - Genes relacionados com estresse são induzidos pela luz visível durante o crescimento micelial, resultando em aumento da tolerância de conídios a condições de estresse
• Beneficiário: Drauzio Eduardo Naretto Rangel
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/06374-1 - Luz visível durante o crescimento induz aumento de tolerância de conídios a diferentes condições de estresse em fungos
• Beneficiário: Drauzio Eduardo Naretto Rangel
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores