| Texto completo | |
| Autor(es): |
De Abreu, Dilayla K.
;
Monteiro, Janaina M.
;
Souza, Carolina C.
;
Karam, Rafael G.
;
Fernandes, Renata A.
;
Lessa, Thais B.
;
Fagliari, Jose J.
;
Miglino, Maria Angelica
;
Ambrosio, Carlos Eduardo
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Anais da Academia Brasileira de Ciências; v. 90, n. 3, p. 14-pg., 2018-07-01. |
| Resumo | |
Duchenne Muscular Dystrophy (DMD) is the most common X-linked muscular disease affecting humans. The Golden Retriever Muscular Dystrophy model (GRMD) is consider the most suitable for several studies. This assay aims to quantify lymphocyte subpopulations CD4, CD5, and CD8, and standardize, the serum electrophoretic profile, to understand their contribution to the pathologic process in normal Golden Retriever dogs (GR group) and dystrophic's (GRMD group), through the umbilical cord blood, in dogs aged from 2 to 3 months (GR II and GRMD II), and in dogs over 1 year of age (GR III and GRMD III). No significant differences were observed between the CD8+ lymphocyte subpopulations of the groups studied. The CD4+ and CD5+ lymphocyte subpopulations were significantly higher in the GRMD III group compared to the GR III group. Twenty-two different proteins in the gel were identified. The serum concentrations of the proteins belonging to the GR I and GRMD I groups were significantly lower than those of the other groups. We show that expression of acute phase proteins are worst during the aging of the dogs. We hope to expand knowledge to better understand the GRMD model and the translational data. (AU) | |
| Processo FAPESP: | 07/51222-2 - Interação de terapia celular e gênica para a correção da distrofia muscular de Duchenne utilizando endonucleases quiméricas |
| Beneficiário: | Carlos Eduardo Ambrósio |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 09/02958-1 - Padronização do perfil hematológico, bioquímico, proteinograma sérico e imunofenotipagem de linfócitos de cães da raça Golden Retrivier sadios e afetados pela distrofia muscular. |
| Beneficiário: | Dilayla Kelly de Abreu |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 13/09392-9 - Produção de células-tronco de pluripotência induzida como modelo de estudo em coelhos |
| Beneficiário: | Carlos Eduardo Ambrósio |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/13030-0 - Imunomodulação in vitro das células tronco mesênquimais em cães da raça Golden Retriever sadios e afetados pela distrofia muscular. |
| Beneficiário: | Dilayla Kelly de Abreu |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |