Exposure to decabromodiphenyl ether (BDE-209) produces mitochondrial dysfunction in rat liver and cell death

Autor(es)

Pereira, Lilian C. ; Souza, Alecsandra O. ; Tasso, Maria J. ; Oliveira, Alana M. C. ; Duarte, Filipe V. ; Palmeira, Carlos M. ; Dorta, Daniel J.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 80, n. 19-21, p. 16-pg., 2017-01-01.

Resumo

Polybrominated diphenyl ethers (PBDE) are ubiquitous environmental pollutants. Exposure to these chemicals has been associated with developmental neurotoxicity, endocrine dysfunctions, reproductive disorders, and hepatotoxicity. The widespread use of PBDE as flame retardants has culminated in daily exposure of humans and wildlife to these contaminants and resulted in their banned use. Thus assessment of the potential effects of each PBDE congener on living organisms has become cause for concern. The aim of this study was to (1) examine the effects of decabromodiphenyl ether (BDE)-209 on different functions of HepG2 cells and (2) investigate whether this congener is involved in mitochondrial toxicity. The use of multiple methods was employed to (i) study the influence of BDE-209 on mitochondrial permeability transition (MPT) process in mitochondria isolated from rat liver and (ii) determine the consequential cellular damage. Our results showed that BDE-209 induced matrix swelling related to MPT with 10 mu M and ATP depletion with 0.1 mu M. In addition, 0.5 mu M BDE-209 reduced HepG2 cell viability, produced collapse of membrane potential, but increased levels of reactive oxygen species (ROS) after 48 h incubation. After 24 h with 5 mu M treatment elevated levels of ROS, DNA fragmentation and cytochrome c release, accompanied by caspase 9 and caspase 3 activation was noted. Taken together, these results suggest that short-duration exposure (24 or 48 h) to 0.5 mu M or 5 mu M BDE-209 concentrations diminished HepG2 cell viability due to apoptosis associated with mitochondrial dysfunction. (AU)

• Processo FAPESP: 12/13123-0 - Avaliação dos congêneres BDE-100 e BDE-153 de éteres difenílicos polibromados sobre a linhagem celular HepG2 e linfócitos humanos: efeitos citotóxicos, genotóxicos e mutagênicos
• Beneficiário: Lilian Cristina Pereira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/06912-6 - Avaliação das alterações mitocondriais e celulares induzidas por representantes das principais classes de bifenilas poli-bromadas (PBDEs)
• Beneficiário: Daniel Junqueira Dorta
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 10/02661-6 - Avaliação das alterações mitocondriais induzidas por representantes das principais classes de bifenilas poli-bromadas (PBDEs)
• Beneficiário: Lilian Cristina Pereira
• Modalidade de apoio: Bolsas no Brasil - Mestrado