Differential expression of RNA exosome subunits in the amphibian Lithobates catesbeianus during reproductive and non-reproductive periods

Autor(es)

Luz, J. S. ; Caneguim, B. H. ; Baggio, A. ; Santoni, M. M. ; Helbing, C. C. ; Valentini, S. R. ; Sasso-Cerri, E. ; Oliveira, C. C.

• Tipo de documento: Artigo Científico
• Fonte: BMC RESEARCH NOTES; v. 12, p. 7-pg., 2019-01-21.

Resumo

Objective: The RNA exosome is an evolutionarily conserved 3'-5' exoribonucleolytic protein complex involved in processing and degradation of different classes of nuclear and cytoplasmic RNAs, and, therefore, important for the posttranscriptional control of gene expression. Despite the extensive in vivo functional studies and the structural data on the RNA exosome, few studies have been performed on the localization and expression of exosome subunits during gametogenesis, process during which gene expression is largely controlled at the posttranscriptional level. Results: We report the identification of exosome subunits in Lithobates catesbeianus and analysis of the differential subcellular localization of RNA exosome core and catalytic subunits in testis cells. In addition, we show seasonal differences in the expression levels of four exosome subunits in different organs. In addition to being part of the RNA exosome complex, its subunits might participate independently of the complex in the control of gene expression during seasonal variation in bullfrog tissues. These results may be relevant for other eukaryotic species. (AU)

• Processo FAPESP: 12/51200-7 - Identificação e determinação da localização celular das subunidades do exossomo em testículos de Lithobates catesbeianus (rã touro)
• Beneficiário: Juliana Silva da Luz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/23845-3 - Avaliação do efeito da Vitamina B12 sobre as fases mitótica e meiótica da espermatogênese e sobre o DNA espermático de ratos tratados com cimetidina
• Beneficiário: Estela Sasso Cerri
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/21383-8 - Avaliação morfofuncional de testículos de rãs touros: uma correlação com os corpos adiposos e com a sazonalidade
• Beneficiário: Andressa Baggio Dias
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/51842-3 - SMOLBNET2: estudo de estrutura de proteínas componentes e reguladoras do exossomo de Archaea e de levedura
• Beneficiário: Carla Columbano de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático