LC-ESI-IT-MS/MS and MALDI-TOF Approach: Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties

Autor(es)

de Souza Mesquita, Leonardo Mendes ; Bittencourt Rodrigues, Caroline Fabri ; da Rocha, Claudia Quintino ; Bianchim, Mayara Silveira ; Rodrigues, Clenilson Martins ; de Oliveira, Vanda Maria ; Gaeta, Henrique Hessel ; Belchor, Mariana Novo ; Toyama, Marcos Hikari ; Vilegas, Wagner

• Tipo de documento: Artigo Científico
• Fonte: NATURAL PRODUCTS AND BIOPROSPECTING; v. 9, n. 1, p. 12-pg., 2019-02-01.

Resumo

We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis [liquid chromatography (LC)-ESI-IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by formalin and tail-flick experimental assays. Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds. The first series of experiments involved [LC]-FIA-IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3 (DP3). The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series: one based on polymers of m/z 288 Da increments (up to DP12) and another series based on polymers of m/z [288 + 162] Da increments (up to DP11). In addition to these series of flavan-3-ol, each DP had a subset of masses with a variation of - 16 Da (homologous series of afzelechins-m/z 873-3465 Da) and + 16 Da (homologous series of gallocatechins-m/z 905-3497 Da). A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series: glucogallocatechins (m/z 779-3371) and glucoafzelechins (m/z 747-3339). The intraperitoneal administration of different doses of AERM (50, 150 and 300 mu g mL(-1)) have a morphine-like effect and intense anti-inflammatory activity. (AU)

• Processo FAPESP: 09/52237-9 - Fitoterápicos padronizados como alvo para o tratamento de doenças crônicas
• Beneficiário: Wagner Vilegas
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 14/23951-3 - Fitoterápicos padronizados para o tratamento de doenças crônicas: Rhizophora mangle
• Beneficiário: Leonardo Mendes de Souza Mesquita
• Modalidade de apoio: Bolsas no Brasil - Mestrado