| Texto completo | |
| Autor(es): |
de Souza Mesquita, Leonardo Mendes
;
Bittencourt Rodrigues, Caroline Fabri
;
da Rocha, Claudia Quintino
;
Bianchim, Mayara Silveira
;
Rodrigues, Clenilson Martins
;
de Oliveira, Vanda Maria
;
Gaeta, Henrique Hessel
;
Belchor, Mariana Novo
;
Toyama, Marcos Hikari
;
Vilegas, Wagner
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | NATURAL PRODUCTS AND BIOPROSPECTING; v. 9, n. 1, p. 12-pg., 2019-02-01. |
| Resumo | |
We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis [liquid chromatography (LC)-ESI-IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by formalin and tail-flick experimental assays. Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds. The first series of experiments involved [LC]-FIA-IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3 (DP3). The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series: one based on polymers of m/z 288 Da increments (up to DP12) and another series based on polymers of m/z [288 + 162] Da increments (up to DP11). In addition to these series of flavan-3-ol, each DP had a subset of masses with a variation of - 16 Da (homologous series of afzelechins-m/z 873-3465 Da) and + 16 Da (homologous series of gallocatechins-m/z 905-3497 Da). A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series: glucogallocatechins (m/z 779-3371) and glucoafzelechins (m/z 747-3339). The intraperitoneal administration of different doses of AERM (50, 150 and 300 mu g mL(-1)) have a morphine-like effect and intense anti-inflammatory activity. (AU) | |
| Processo FAPESP: | 09/52237-9 - Fitoterápicos padronizados como alvo para o tratamento de doenças crônicas |
| Beneficiário: | Wagner Vilegas |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Temático |
| Processo FAPESP: | 14/23951-3 - Fitoterápicos padronizados para o tratamento de doenças crônicas: Rhizophora mangle |
| Beneficiário: | Leonardo Mendes de Souza Mesquita |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |