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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A comparative study of the purification of betanin

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Autor(es):
Pires Goncalves, Leticia Christina [1] ; de Souza Trassi, Marco Aurelio [1] ; Lopes, Nathana Barbosa [1] ; Doerr, Felipe Augusto [2] ; dos Santos, Monica Teixeira [1] ; Baader, Wilhelm Josef [3] ; Oliveira, Jr., Vani Xavier [1] ; Bastos, Erick Leite [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Food Chemistry; v. 131, n. 1, p. 231-238, MAR 1 2012.
Citações Web of Science: 44
Resumo

Betanin is a natural pigment with antioxidant properties used as a food colourant. This work describes the spectrophotometric and chromatographic quantification of betanin (2S/15S) and its epimer isobetanin (2S/15R) in fresh beetroot juice, food-grade beetroot powder and betanin standard diluted in dextrin. Absorption spectra of all three samples were deconvoluted using a mixed three-function model. Food-grade beetroot powder has the largest amount of violet-red impurities, probably formed during processing. The purification of betanin from these complex matrices was carried out by seven different methods. Ion exchange chromatography was the most efficient method for the purification of betanin from all samples; however, fractions contain high amounts of salt. Reversed-phase HPLC as well as reversed-phase column chromatography also produced good results at a much faster rate. The longer retention time of isobetanin when compared to betanin in reversed-phase conditions has been investigated by means of quantum-mechanical methods. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 07/00684-6 - Síntese e aplicação de derivados de pigmentos de flores
Beneficiário:Erick Leite Bastos
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 07/59407-1 - Betalaínas semissintéticas como antioxidantes e pigmentos biocompatíveis
Beneficiário:Leticia Christina Pires Gonçalves
Linha de fomento: Bolsas no Brasil - Doutorado Direto