| Full text | |
| Author(s): |
Pires Goncalves, Leticia Christina
[1]
;
de Souza Trassi, Marco Aurelio
[1]
;
Lopes, Nathana Barbosa
[1]
;
Doerr, Felipe Augusto
[2]
;
dos Santos, Monica Teixeira
[1]
;
Baader, Wilhelm Josef
[3]
;
Oliveira, Jr., Vani Xavier
[1]
;
Bastos, Erick Leite
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Food Chemistry; v. 131, n. 1, p. 231-238, MAR 1 2012. |
| Web of Science Citations: | 44 |
| Abstract | |
Betanin is a natural pigment with antioxidant properties used as a food colourant. This work describes the spectrophotometric and chromatographic quantification of betanin (2S/15S) and its epimer isobetanin (2S/15R) in fresh beetroot juice, food-grade beetroot powder and betanin standard diluted in dextrin. Absorption spectra of all three samples were deconvoluted using a mixed three-function model. Food-grade beetroot powder has the largest amount of violet-red impurities, probably formed during processing. The purification of betanin from these complex matrices was carried out by seven different methods. Ion exchange chromatography was the most efficient method for the purification of betanin from all samples; however, fractions contain high amounts of salt. Reversed-phase HPLC as well as reversed-phase column chromatography also produced good results at a much faster rate. The longer retention time of isobetanin when compared to betanin in reversed-phase conditions has been investigated by means of quantum-mechanical methods. (C) 2011 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 07/59407-1 - Semi-synthetic betalains as biocompatible antioxidants and pigments |
| Grantee: | Leticia Christina Pires Gonçalves |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 07/00684-6 - Synthesis and application of modified flower pigments |
| Grantee: | Erick Leite Bastos |
| Support Opportunities: | Research Grants - Young Investigators Grants |