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Bioprospection of animal toxins with biotechnological interest through omic tools

Grant number: 15/18432-0
Support type:Regular Research Grants
Duration: September 01, 2016 - February 28, 2019
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal Investigator:Eliane Candiani Arantes Braga
Grantee:Eliane Candiani Arantes Braga
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Assoc. researchers: Carlos Ueira-Vieira ; Renata Santos Rodrigues
Associated grant(s):16/50472-4 - Trans Atlantic Venomics (TRAVIX), AP.R SPRINT

Abstract

Poisons and venoms are source of biologically active molecules that can be used as medicine. Despite the great variety of molecules with biotechnological application, it is still difficult to develop them, due to the small amount of starting material, low purification yield and high cost of the traditional purification methodology, which explains the small number of animal molecules currently used as drugs. Therefore, the advent and further increment of omic tools are paving the new state of art, once its use allows the researches to have an overview of the whole venom or poison composition. In addition, the transcriptome technique associated with cloning and heterologous expression enables the production of enough quantity of molecules from the gland or specialized tissue for structural and functional analysis. Therefore, these studies facilitate the use of molecules with significant biological activity. In order to validate the activity of recombinant proteins, structural, functional and comparative tests between native and recombinant molecules are relevant and may be designed to study functional and structural characteristics. Therefore, this project aims the bioprospection toxins with potential application of neglected species. Several assays will be performed for this purpose, as transcriptomes and proteomes construction, mass fingerprinting assays, cloning and expression of heterologous proteins and functional and structural tests for native toxins from R. schneideri toad and P. villosa ant C. d. terrificus snake, and scorpion secretions. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BOLDRINI-FRANCA, JOHARA; PINHEIRO-JUNIOR, ERNESTO LOPES; PEIGNEUR, STEVE; PUCCA, MANUELA BERTO; CERNI, FELIPE AUGUSTO; BORGES, RAFAEL JUNQUEIRA; COSTA, TASSIA RAFAELLA; IMAI CARONE, SANTE EMMANUEL; DE MATTOS FONTES, MARCOS ROBERTO; SAMPAIO, SUELY VILELA; ARANTES, ELIANE CANDIANI; TYTGAT, JAN. Beyond hemostasis: a snake venom serine protease with potassium channel blocking and potential antitumor activities. SCIENTIFIC REPORTS, v. 10, n. 1 MAR 11 2020. Web of Science Citations: 1.
WIEZEL, GISELE A.; BORDON, KARLA C. F.; SILVA, RONIVALDO R.; GOMES, MARIO S. R.; CABRAL, HAMILTON; RODRIGUES, VERIDIANA M.; UEBERHEIDE, BEATRIX; ARANTES, ELIANE C. Subproteome of Lachesis muta rhombeata venom and preliminary studies on LmrSP-4, a novel snake venom serine proteinase. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 25, APR 15 2019. Web of Science Citations: 0.
TANAKA SHIBAO, PRISCILA YUMI; COLOGNA, CAMILA TAKENO; MORANDI-FILHO, ROMUALDO; WIEZEL, GISELE ADRIANO; FUJIMURA, PATRICIA TIEMI; UEIRA-VIEIRA, CARLOS; ARANTES, ELIANE CANDIANI. Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretion. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, NOV 29 2018. Web of Science Citations: 1.
WIEZEL, GISELE A.; SHIBAO, PRISCILA Y. T.; COLOGNA, CAMILA T.; MORANDI FILHO, ROMUALDO; UEIRA-VIEIRA, CARLOS; DE PAUW, EDWIN; QUINTON, LOIC; ARANTES, ELIANE C. In-Depth Venome of the Brazilian Rattlesnake Crotalus durissus terrificus: An Integrative Approach Combining Its Venom Gland Transcriptome and Venom Proteome. JOURNAL OF PROTEOME RESEARCH, v. 17, n. 11, p. 3941-3958, NOV 2018. Web of Science Citations: 2.
COLOGNA, CAMILA TAKENO; RODRIGUES, RENATA SANTOS; SANTOS, JEAN; DE PAUW, EDWIN; ARANTES, ELIANE CANDIANI; QUINTON, LOIC. Peptidomic investigation of Neoponera villosa venom by high-resolution mass spectrometry: seasonal and nesting habitat variations. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, p. -, 2018. Web of Science Citations: 4.
SANTUSSI, WELLIGTON M.; BORDON, KARLA C. F.; RODRIGUES ALVES, ANA P. N.; COLOGNA, CAMILA T.; SAID, SURAIA; ARANTES, ELIANE C. Antifungal Activity against Filamentous Fungi of Ts1, a Multifunctional Toxin from Tityus serrulatus Scorpion Venom. FRONTIERS IN MICROBIOLOGY, v. 8, JUN 6 2017. Web of Science Citations: 2.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.