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Transcriptome, cloning, expression and evaluation of the biological activities of the main toxins of Cryptops iheringi venom

Grant number: 17/13812-4
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2017
Effective date (End): June 30, 2020
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Geraldo Santana Magalhães
Grantee:Lhiri Hanna Alves de Lucca Shimokawa Falcão
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Chilopoda are a class of venomous arthropods widely distributed throughout the world. Because they are well adapted to live in urban and domestic areas several cases of humans bitten have been reported. Despite the poor medical relevance, human envenomation with centipedes can cause a number of unpleasant symptoms such as burning pain, paresthesia, edema and superficial necrosis at the bite site. Rare cases, progress to a serious condition. A clinical study with patients attended at Hospital Vital Brazil, Butantan Institute, showed that the majority of accidents with centipedes were caused by the genus Cryptops and Otostigmus representing about 90% of the cases. Based upon symptoms induced by centipede envenomation, it has been suggested that the centipede venom comprises a diverse set of proteins, peptides, and enzymes with a variety of biological activities. In this regard, it has been reported in the literature several bioactive compounds, some of which have potential therapeutic interest. However, despite the significant pharmacological importance, very little is known about the active components of the venom of these animals. Therefore, the venom of centipedes can be an excellent source of toxins still unknown and with untapped biotechnological potential. Considering that the genus Cryptops is one of the most associated to accidents in humans and that to the present moment there is no literature on the toxins of its venom, in this project we intend to perform a transcriptomic analysis of the venom gland of Cryptops iheringi in order to obtain a profile of the toxins of this species. The most abundant and interesting biotechnological toxins genes will be expressed in bacteria and their biological activities will be evaluated. (AU)