Antifungal activity of 2 '-hydroxychalcone loaded in nanoemulsion against Paracoccidioides spp.

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Medina-Alarcon, Kaila P. ; Singulani, Junya de L. ; Dutra, Luiz A. ; Pitangui, Nayla de S. ; Pereira-da-Silva, Marcelo A. ; dos Santos, Mariana B. ; Ayusso, Gabriela M. ; Regasini, Luis O. ; Soares, Christiane P. ; Chorilli, Marlus ; Mendes-Giannini, Maria Js ; Fusco-Almeida, Ana M.

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. 15, n. 1, p. 13-pg., 2020-01-01.

Resumo

Aim: This study aimed to evaluate the activity of 2-hydroxychalcone-loaded in nanoemulsion (NLS + 2 ' chalc), the cytotoxic effect and toxicity against Paracoccidioides brasiliensis and Paracoccidioides lutzii using a zebrafish model. Materials & methods: Preparation and physical-chemical characterization of nanoemulsion (NLS) and NLS + 2 ' chalc were performed. MIC and minimum fungicide concentration, cytotoxicity and toxicity were also evaluated in the Danio rerio model. Results: NLS + 2 ' chalc showed fungicidal activity against Paracoccidioides spp. without cytotoxicity in MRC5 and HepG2 lines. It also had high selectivity index values and no toxicity in the zebrafish model based on MIC values. Conclusion: NLS + 2 ' chalc is a potential new alternative treatment for paracoccidioidomycosis. Graphical abstract (AU)

• Processo FAPESP: 16/05581-0 - Desenvolvimento de protótipo antifúngico nanoestruturado lipídico contra biofilmes de Paracoccidioides brasiliensis e Paracoccidioides lutzii e avaliação da toxicidade em modelo animal zebrafish
• Beneficiário: Giovana Garcia Ferin
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica