Characterization of a beta-amylase from Propionicimonas sp ENT-18 ectosymbiont of Acromyrmex subterraneus brunneus

Autor(es)

Zucchi, Tiago Domingues ^[1] ; Rossi, Guilherme D. ^[1] ; Consoli, Fernando L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Entomol & Acarol, Escola Super Agr Luiz de Queiroz, Lab Interacoes Insetos, BR-13418900 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANNALS OF MICROBIOLOGY; v. 61, n. 4, p. 985-990, DEC 2011.
• Citações Web of Science: 2

Resumo

The last decade has seen an increase in the industrial use of amylase, mainly due to its role in the production of anhydrous ethanol through the hydrolysis of corn starch. Most amylases in use nowadays are isolated from microorganisms, indicating their potential as a source of amylases for industrial applications. Since most microbial diversity remains unknown, microbes associated with previously undiscovered niches can provide an untapped source of new molecules. We investigated the diversity of actinobacteria associated with an underexploited niche and have identified a number of rare actinobacteria. We further aimed to characterize the amylase produced by one such actinobacterium, Propionicimonas sp. ENT-18, an ectosymbiont associated with the integument of Acromyrmex subterraneus brunneus. Our data indicate that this actinobacterium produces a high-molecular-weight beta-amylase (170.4 kDa), with maximum production at the end of the log phase (96 h at 28 degrees C) when cultured in Starch-Czapek broth. The maximum specific activity (50 mU/mg protein) was obtained at pH 5.5 at 30 degrees C. The beta-amylase produced was partially purified by ice-cold acetone precipitation, with a recovery of 65% activity. Although this enzyme has not shown any special trait (e. g., thermal or pH stability) for industrial applications, this is the first report of a beta-amylase-producing Propionicimonas. (AU)

• Processo FAPESP: 07/59361-1 - Avaliação do cDNA de uma quitinase isolado da interação Heliothis virescens-Toxoneuron nigriceps no desenvolvimento de método de controle de pragas
• Beneficiário: Guilherme Duarte Rossi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/58712-5 - Actinobactérias simbiontes de Acromyrmex sp.: fonte potencial de novas biomoléculas para o controle de pragas e doenças
• Beneficiário: Tiago Domingues Zucchi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado