High Trypanosoma vivax infection rates in water buffalo and cattle in the Brazilian Lower Amazon

Autor(es)

Garcia Perez, Herakles Antonio ; Filgueira Rodrigues, Carla Monadeli ; Vivas Pivat, Isis Helga ; Rodrigues Fuzato, Adriana Carlos ; Camargo, Erney P. ; Hamad Minervino, Antonio Humberto ; Geraldes Teixeira, Marta Maria

• Tipo de documento: Artigo Científico
• Fonte: Parasitology International; v. 79, p. 9-pg., 2020-12-01.

Resumo

Highly sensitive and accurate molecular diagnostic methods have not yet been employed for livestock trypanosomosis in the Brazilian Lower Amazon although the first reports of Trypanosoma vivax and Trypanosoma evansi in Brazil were in water buffalo (Bubalus bubalis) in this region. The present study assessed trypanosomosis in buffalo and cattle raised in communal and seasonally flooding pastures in the state of Para using the fluorescent fragment length barcoding (FFLB) method. T. evansi was not detected, but high infection rates of T. vivax and T. theileri were revealed by a simplified FFLB standardized in the present study that discriminates all trypanosome species infective to livestock in South America. T. vivax infection rates detected by TviCATL-PCR were 24.6% for cattle (n = 61) and 28.1% for buffalo (n = 89). Using the FFLB method, overall T. vivax infection rates increased to 59.6% and 44.3% for buffalo and cattle, respectively. Furthermore, the predominance of a single microsatellite-based genotype of T. vivax was reinforced in the Lower Amazon. Relevant T. vivax infection rates detected in clinically healthy buffalo and cattle through the sampled years (2008-2017) highlight the need for systematic studies to demonstrate the endemic steady state of T. vivax in this region. Our findings provide baseline information for livestock management, including control of T. vivax dispersal, and the introduction of naive animals. The growing international trade of live livestock from this very important livestock breeding region represents a serious risk for T. vivax spreading outside Amazonia and Brazil. (AU)

• Processo FAPESP: 16/07487-0 - Ampliação e codificação (DNA barcoding) da coleção de culturas de tripanossomatídeos da USP (TCC-USP) e sua utilização em estudos filogenéticos e taxonômicos
• Beneficiário: Erney Felicio Plessmann de Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/03028-1 - Estudo comparado de tripanossomas de ungulados da África e América do Sul: diversidade, diagnóstico, filogenia e epidemiologia molecular
• Beneficiário: Herakles Antonio Garcia Perez
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado