Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry

Autor(es)

Contesini, Fabiano Jares ; Davanco, Marcelo Gomes ; Boriin, Gustavo Pagotto ; Vanegas, Katherina Garcia ; Goncalves Cirino, Joao Pedro ; de Melo, Ricardo Rodrigues ; Mortensen, Uffe Hasbro ; Hilden, Kristiina ; Campos, Daniel Rossi ; Carvalho, Patricia de Oliveira

• Tipo de documento: Artigo Científico
• Fonte: CATALYSTS; v. 10, n. 9, p. 33-pg., 2020-09-01.

Resumo

Lipases are one of the most used enzymes in the pharmaceutical industry due to their efficiency in organic syntheses, mainly in the production of enantiopure drugs. From an industrial viewpoint, the selection of an efficient expression system and host for recombinant lipase production is highly important. The most used hosts areEscherichia coliandKomagataella phaffii(previously known asPichia pastoris) and less often reportedBacillusandAspergillusstrains. The use of efficient expression systems to overproduce homologous or heterologous lipases often require the use of strong promoters and the co-expression of chaperones. Protein engineering techniques, including rational design and directed evolution, are the most reported strategies for improving lipase characteristics. Additionally, lipases can be immobilized in different supports that enable improved properties and enzyme reuse. Here, we review approaches for strain and protein engineering, immobilization and the application of lipases in the pharmaceutical industry. (AU)

• Processo FAPESP: 18/13317-6 - Estudo lipidômico aplicado ao Câncer de Reto: mecanismos bioquímicos-moleculares e avaliação da resposta à terapia neoadjuvante
• Beneficiário: Patrícia de Oliveira Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/14253-9 - Avaliação do potencial biotecnológico do sistema celulolítico de Xanthomonas axonopodis pv. citri: uma abordagem estrutural, funcional e aplicada
• Beneficiário: Ricardo Rodrigues de Melo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado