Thermodynamic profile and molecular modeling of the interaction between Grb2 dimer and flavonoids Rutin and Morin

Autor(es)

Sanches, Karoline ; Dias, Raphael V. R. ; da Silva, Paulo H. ; Caruso, Icaro P. ; Fossey, Marcelo A. ; de Souza, Fatima P. ; de Oliveira, Leandro C. ; Melo, Fernando A.

• Tipo de documento: Artigo Científico
• Fonte: Journal of Molecular Structure; v. 1234, p. 10-pg., 2021-03-06.

Resumo

The adaptor protein growth factor-bound protein 2 (Grb2) is an important regulator of the fibroblast growth factor receptor 2 (FGFR2) before extracellular stimuli. It is known to form complexes that end up in the mitogen-activated protein kinase (MAPK) pathway activation, which is involved in proliferation and oncogenic signal transduction. Grb2 is a versatile protein performing functions other than adaptor protein, making it a relevant target to verify its interaction with flavonoids such as Rutin and Morin. These small polyphenols molecules are easy to be found in the nature and its anti-tumor properties are well-known. By using fluorescence spectroscopy, the thermodynamic profile of the interaction between those molecules and Grb2 showed entropically driven interactions, where hydrophobic effects take place as the main interaction potential. The dissociation constants found were K-d similar to 10(-6) M for Morin and K-d similar to 10(-5) M for Rutin. The molar ratio protein/ligand is 1:1 for both assays. Furthermore, nuclear magnetic resonance has provided important information about the protein-ligand interaction epitopes, which has been used as a guide for molecular docking and molecular dynamics simulations. The combination of the obtained results shows the SH2 domain as the most probable interaction place on Grb2 dimer for Rutin and Morin binding. Sh2 is a well-known domain responsible for pY (phosphotyrosine) recognition upon protein partners and an important protein module for testing SH2 domain inhibitors. (C) 2021 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 16/08753-6 - Avaliação de mecanismos de quinases e proteínas relacionadas
• Beneficiário: Raphael Vinicius Rodrigues Dias
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/17630-0 - Estudos funcionais da tirosina quinase Fibroblast Growth Factor receptor (FGFR2), da adaptadora growth factor Receptor- bound protein 2 (GRB2) e da tirosina fosfatase SHP2
• Beneficiário: Fernando Alves de Melo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/24974-0 - Estudos funcionais da proteína growth-factor-receptor bound protein 2 (GRB2) por meio de ressonância magnética nuclear e fluorescência: correlação entre dinâmica e estrutura
• Beneficiário: Fernando Alves de Melo
• Modalidade de apoio: Auxílio à Pesquisa - Regular