Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitis

Autor(es):Mostrar menos -

Lisa Danielly Curcino ARAUJO ^[1] ; Raquel Assed Bezerra SEGATO ^[2] ; Thaís de Paula Colen REIS ^[3] ; Sérgio Luis de Souza SALVADOR ^[4] ; Flávia Aparecida Chaves FURLANETO ^[5] ; Michel Reis MESSORA ^[6] ; Paulo NELSON-FILHO ^[7] ; Lana Kei Yamamoto de ALMEIDA ^[8] ; Marília Pacífico LUCISANO ^[9] ; Clara Marina Pereira Cavalcanti SILVA ^[10] ; Léa Assed Bezerra da SILVA ^[11]

Afiliação do(s) autor(es):Mostrar menos -

^[1] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[2] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[3] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[4] Universidade de São Paulo. School of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses - Brasil

^[5] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Oral and Maxillofacial Surgery and Periodontology - Brasil

^[6] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Oral and Maxillofacial Surgery and Periodontology - Brasil

^[7] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[8] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[9] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[10] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[11] Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Oral Research; v. 37, 2023-11-10.

Resumo

Abstract This study aimed to evaluate the effect of Bifidobacterium animalis subsp. lactis (B. lactis) HN019 in drinking water on the development of apical periodontitis (AP) in rats. In total 60 animals were divided into a control group (sound teeth); Group I - regular water without AP; Group II - probiotic water without AP; Group III - regular water with AP; Group IV - probiotic water with AP. AP was induced after 3 days in the control groups and after 7, 21, and 42 days in groups III and IV. The animals were euthanized, and the mandibles were subjected to histotechnical processing. Samples were stained with hematoxylin & eosin (H&E) to identify root canal features, apical and periapical regions. Additionally, histoenzymology was performed to detect osteoclasts, immunohistochemistry was used to identify osteoclastogenesis markers, and the Brown & Brenn technique was applied for microbiological analysis. The data were analyzed using GraphPad Prism 8.0.1 with a significance level of 5%. Although no statistical differences were observed, the groups administered with probiotics showed better conditions in terms of histological aspects seen microscopically. Furthermore, there were no differences in the number of osteoclasts (p > 0.05). The RANKL marker was not found in the probiotic group at 42 days, unlike in group III. (AU)

• Processo FAPESP: 18/22038-3 - Análise microscópica da gênese e progressão de lesões periapicais após o uso sistêmico e local de probiótico em ratos
• Beneficiário: Lisa Danielly Curcino Araujo
• Modalidade de apoio: Bolsas no Brasil - Mestrado