Identification and antimicrobial susceptibility profile of bacteria isolated from primary endodontic infections

Autor(es)

Lorena Souza Santos MARIANO ^[1] ; Rafael NAKAMURA-SILVA ^[2] ; Luciana Martins Domingues de MACEDO ^[3] ; Mariana de OLIVEIRA-SILVA ^[4] ; Rafael da Silva GOULART ^[5] ; Marsileni PELISSON ^[6] ; Eliana Carolina VESPERO ^[7] ; Yara Teresinha Correa SILVA-SOUSA ^[8] ; André PITONDO-SILVA ^[9]

Afiliação do(s) autor(es)

^[1] Universidade de Ribeirão Preto. Postgraduate Program in Dentistry - Brasil

^[2] Universidade de Ribeirão Preto. Postgraduate Program in Environmental Technology - Brasil

^[3] Universidade de Ribeirão Preto. Postgraduate Program in Dentistry - Brasil

^[4] Universidade de Ribeirão Preto. Postgraduate Program in Environmental Technology - Brasil

^[5] Universidade de Ribeirão Preto. Postgraduate Program in Dentistry - Brasil

^[6] Universidade Estadual de Londrina. Department of Pathology, Clinical and Toxicological Analysis - Brasil

^[7] Universidade Estadual de Londrina. Department of Pathology, Clinical and Toxicological Analysis - Brasil

^[8] Universidade de Ribeirão Preto. Postgraduate Program in Dentistry - Brasil

^[9] Universidade de Ribeirão Preto. Postgraduate Program in Dentistry - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Oral Research; v. 38, 2024-04-05.

Resumo

Abstract This study aimed to identify and characterize the antimicrobial susceptibility profile of bacteria found in primary endodontic infections in the teeth of patients treated at the Dental Clinic of the University of Ribeirão Preto, São Paulo, Brazil. From September to December 2019, samples were obtained from 21 patients with primary endodontic infections. The collections were carried out in triplicate using paper cones placed close to the total length of the root canal. Bacterial isolation was performed in Brain Heart Infusion agar, Blood agar, and other selective culture media cultured at 37°C for up to 48 h under aerobiosis and microaerophilic conditions. The bacterial species were identified using the Vitek 2 automated system. The disk diffusion method on agar Müeller–Hinton was used to assess antimicrobial susceptibility with the recommended antimicrobials for each identified bacterial species. A total of 49 antibiotics were evaluated. Fifteen of the 21 samples collected showed bacterial growth, and 17 bacterial isolates were found. There were 10 different bacterial species identified: Enterococcus faecalis (four isolates), Streptococcus mitis/oralis (three isolates), Streptococcus anginosus (three isolates) being the most common, followed by Staphylococcus epidermidis, Enterococcus faecium, Streptococcus constellatus, Streptococcus alactolyticus, Enterobacter cloacae, Klebsiella variicola, and Providencia rettgeri (one isolate of each species). The analysis demonstrated significant susceptibility to most of the tested antibiotics. However, some Enterococcus isolates resisted the antibiotic’s erythromycin, ciprofloxacin, and tetracycline. A Staphylococcus epidermidis isolate was characterized as multidrug-resistant. Five Streptococcus isolates were non-susceptible to all antibiotics tested. (AU)

• Processo FAPESP: 13/22581-5 - Estudo dos mecanismos de patogenicidade e correlação com resistência a antimicrobianos de Klebsiella pneumoniae isoladas no Brasil e em países dos cinco continentes
• Beneficiário: André Pitondo da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores