Mango peel as a potential enzyme inducer in Trichoderma harzianum: a strategy for cariogenic biofilm degradation and reuse of industrial waste

Autor(es)

Jéssica Silva Peixoto Bem ^[1] ; Ana Cristina Morseli Polizello ^[2] ; Hamilton Cabral ^[3] ; Nathalia Gonsales da Rosa-Garzon ^[4] ; Carem Gledes Vargas Rechia ^[5] ; Carolina Patrícia Aires ^[6]

Afiliação do(s) autor(es)

^[1] University of São Paulo. Department of Children›s Clinic - Brasil

^[2] University of São Paulo. Department of BioMolecular Sciences - Brasil

^[3] University of São Paulo. Department of Pharmaceutical Sciences - Brasil

^[4] University of São Paulo. Department of Pharmaceutical Sciences - Brasil

^[5] University of São Paulo. Department of BioMolecular Sciences - Brasil

^[6] University of São Paulo. Department of BioMolecular Sciences - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Braz. J. Oral Sci.; v. 22, 2024-02-02.

Resumo

Water-insoluble exopolysaccharides (I-EPS) are a virulence factor for dental biofilms. It has already been demonstrated that mango pulp induces the secretion of glucan-hydrolytic enzymes in the fungus Trichoderma harzianum, and that they have an effect on I-EPS from young biofilms. Aim Evaluate the effect of mango peel as an enzyme inducer in T. harzianum, and the effect of enzymes secreted on mature biofilms. Methods Fractions of the peel (PL) and ethanol-precipitated pulp (PP) of Tommy Atkins mangoes were sterilized and added to a culture medium containing T. harzianum for induction of hydrolytic enzymes. After 192 h, the culture medium was centrifuged and the supernatant (enzyme extract) was used as treatment on S. mutans biofilms (n=9): a) NaCl 0.9 %; b) 0.12 % chlorhexidine digluconate; and c) extract of enzymes induced by PL or PP. Acidogenicity, bacterial viability, quantification of insoluble polysaccharides, and three-dimensional analysis of the biofilm by scanning electron microscopy (SEM) was performed. Data were analyzed by ANOVA followed by the Tukey test (α=5 %). Results The hydrolytic enzymes did not alter the metabolism or bacterial viability of the biofilm (p<0.05). Although the images obtained by SEM suggest some degree of matrix degradation, the quantification of I-EPS for the PL and PP groups did not differ from the control group (p>0.05), suggesting a slight effect on the disorganization of the mature S. mutans biofilm. Conclusion The results suggest that mango peel fraction can induce secretion of mutanase by T. harzianum, however in an insufficient amount to generate significant degradation on cariogenic biofilm. (AU)

• Processo FAPESP: 19/05832-0 - RESÍDUOS INDUSTRIAIS DE MANGA COMO FONTE PARA INDUÇÃO DE ENZIMAS EM Trichoderma harzianum: POTENCIAL PARA DEGRADAÇÃO DO BIOFILME DENTAL CARIOGÊNICO
• Beneficiário: Carolina Patrícia Aires Garbellini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/07315-0 - Resíduos industriais de manga para controle de biofilme cariogênico: estudo in vitro
• Beneficiário: Jéssica Silva Peixoto Bem
• Modalidade de apoio: Bolsas no Brasil - Mestrado