Selective inhibitory activity of multidrug-resistant bacteria by zinc oxide nanoparticles

Autor(es)

Marques, Gleison N. ; Moreira, Ailton Jose ; Nobrega, Eryka Thamyris D. ; Braga, Sandalene ; Argentin, Marcela N. ; Camargo, Ilana L. B. da Cunha ; Azevedo, Emilio ; Pereira, Ernesto C. ; Bernardi, Maria Ines B. ; Mascaro, Lucia H.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING; v. 12, n. 1, p. 13-pg., 2024-01-13.

Resumo

The emergence of multidrug-resistant bacteria has attracted much attention from the global community due to their potential to cause harm to health. In this context, ZnO nanoparticles (NPs) produced under different synthesis conditions were successfully applied to inhibit several species of multiresistant bacteria from clinical isolates. Five gram-positive (Staphylococcus epidermidis ATCC 35984, Staphylococcus aureus ATCC 25923, S. aureus ATCC 8095, Enterococcus faecalis ATCC 29212, and Enterococcus faecium ATCC 700221) and four gramnegative bacterial isolates (Klebsiella pneumoniae ATCC 700603, Escherichia coli ATCC 25922, Acinetobacter baumannii ATCC 19606, and Pseudomonas aeruginosa ATCC 27853), known to be involved in healthcareassociated infections and multidrug resistance, were selected for this study. Due to their physicochemical properties, we found that ZnO NPs can exhibit selective inhibition. The minimum inhibitory concentration (MIC) for multidrug-resistant bacteria sensitive to ZnO NPS was determined from varying contact time and catalyst concentration from exhaustive tests. Different characterization techniques were studied to determine the crystalline, optical, morphological, and photocatalytic characteristics of ZnO NPs. The bacterial inhibition mechanisms were discussed in detail with support of photocatalytic assays and cytotoxicity of plant species. In addition, ZnO NPs have also shown great potential for application in the environmental remediation of ecosystems contaminated by organic pollutants due to their efficiency in degrading the dye methylene blue (MB) under UV light with a photocatalytic efficiency of 75% in 60 min. The ZnO NPs showed great potential for multidrugresistant bacteria, with MICs ranging from 256 to 512 mg/L. This demonstrates that the ZnO NPs produced here have the potential to be used as a technology with great application potential in materials used in hospital environments to mitigate the growth and development of these classes of multi-resistant bacteria. (AU)

• Processo FAPESP: 22/06219-3 - Desenvolvendo e disseminando novas tecnologias para remediação ambiental de contaminantes emergentes
• Beneficiário: Ailton Jose Moreira
• Modalidade de apoio: Auxílio à Pesquisa - Projeto Geração
• Processo FAPESP: 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais
• Beneficiário: Elson Longo da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 21/06128-5 - Estudo da atividade antiviral contra SARS-CoV-2 de nanopartículas de cobre e óxido de cobre suportadas em kefiran
• Beneficiário: Gleison Neres Marques
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 22/05254-0 - Redução eletroquímica de dióxido de carbono visando a síntese de precursores de materiais poliméricos
• Beneficiário: Eryka Thamyris Damascena Nobrega Cavalcanti
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos
• Beneficiário: Glaucius Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 22/07984-5 - Centro de Pesquisa e Inovação em Biodiversidade e Fármacos
• Beneficiário: Marcela Nunes Argentin
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico